BME103 s2013:TEMPwu3

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(New System: Machine/ Device Engineering)
(Protocols)
Line 146: Line 146:
<br><br>
<br><br>
-
==Protocols==
+
==New System: Protocols==
-
<!--- Design a new protocol based on your group's new PCR design. Make a step-by-step list of how someone should use your method
+
'''DESIGN AND MATERIALS'''
-
Things to consider:
+
<!-- Design a new protocol based on your group's new PCR design. Things to consider:
How should the PCR machine be set up? Does it need to be plugged in?
How should the PCR machine be set up? Does it need to be plugged in?
How many samples will fit into a single 2-hour run?
How many samples will fit into a single 2-hour run?
Line 157: Line 157:
If it is separate, you will need to include instructions on how to use the fluorimeter.
If it is separate, you will need to include instructions on how to use the fluorimeter.
How should the user calculate the about of signal amplified?
How should the user calculate the about of signal amplified?
-
etc.
+
etc. -->
-
--->
+
-
'''Materials'''
+
'''MATERIALS'''
<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
-
'''PCR Protocol'''
+
'''PCR PROTOCOL'''
 +
<!-- Create a step-by-step procedure for setting up and running PCR reactions. Your instructions should include everything from adding reagents to the tubes, to programming the PCR machine and running the reaction.
Line 171: Line 171:
'''DNA Measurement Protocol'''
'''DNA Measurement Protocol'''
-
 
-
 
-
 
==Research and Development==
==Research and Development==

Revision as of 19:41, 9 April 2013

BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png

Contents

OUR TEAM

Name: StudentRole(s)
Name: Student
Role(s)
Name: StudentRole(s)
Name: Student
Role(s)
Name: StudentRole(s)
Name: Student
Role(s)
Name: StudentRole(s)
Name: Student
Role(s)
Name: StudentRole(s)
Name: Student
Role(s)

LAB 3 WRITE-UP

Original System: PCR Results

PCR Test Results

Sample Name ng/mL Conclusion (pos/neg)
Positive Control --- N/A
Negative Control --- N/A
Tube Label:___ Patient ID: ____ rep 1 ---
Tube Label:___ Patient ID: ____ rep 2 ---
Tube Label:___ Patient ID: ____ rep 3 ---
Tube Label:___ Patient ID: ____ rep 1 ---
Tube Label:___ Patient ID: ____ rep 2 ---
Tube Label:___ Patient ID: ____ rep 3 ---


Bayesian Statistics
These statistics are based upon all of the DNA detection system results that were obtained in the PCR lab for 34 hypothetical patients who were diagnosed as either having cancer or not having cancer.

Bayes Theorem equation: P(A|B) = P(B|A) * P(A) / P(B)


Calculation 1: The probability that the sample actually has the cancer DNA sequence, given a positive diagnostic signal.

  • A = [text description] = [frequency shown as a fraction] = [final numerical value]
  • B = [text description] = [frequency shown as a fraction] = [final numerical value]
  • P (B|A) = [text description] = [frequency shown as a fraction] = [final numerical value]
  • P(A|B) = [answer]



Calculation 3: The probability that the patient will develop cancer, given a cancer DNA sequence.

  • A = [text description] = [frequency shown as a fraction] = [final numerical value]
  • B = [text description] = [frequency shown as a fraction] = [final numerical value]
  • P (B|A) = [text description] = [frequency shown as a fraction] = [final numerical value]
  • P(A|B) = [answer]



New System: Design Strategy

We concluded that a good system Must Have:

  • [Must have #1 - why? short, ~4 or 5 sentences]
  • [Must have #2 - why? short, ~4 or 5 sentences]


We concluded that we would Want a good system to have:

  • [Want #1 - why? short, ~4 or 5 sentences]
  • [Want #2 - why? short, ~4 or 5 sentences]


We concluded that a good system Must Not Have:

  • [Must Not Have #1 - why? short, ~4 or 5 sentences]
  • [Must Not Have #2 - why? short, ~4 or 5 sentences]


We concluded that a good system Should Avoid:

  • [Should Avoid #1 - why? short, ~4 or 5 sentences]
  • [Should Avoid #2 - why? short, ~4 or 5 sentences]




New System: Machine/ Device Engineering

SYSTEM DESIGN


KEY FEATURES

We chose to include these new features

  • Feature 1 - explanation of how this addresses any of the specifications in the "New System: Design Strategy" section
  • Feature 2 - explanation of how this addresses any of the specifications in the "New System: Design Strategy" section
  • Etc.

[OR]

We chose keep the devices the same as the original system

  • Feature 1 - explanation of how a pre-existing feature addresses any of the specifications in the "New System: Design Strategy" section
  • Feature 2 - explanation of how a pre-existing feature addresses any of the specifications in the "New System: Design Strategy" section
  • Etc.


INSTRUCTIONS




New System: Protocols

DESIGN AND MATERIALS

MATERIALS


PCR PROTOCOL


Background on Disease Markers



Primer Design



Illustration




Personal tools