SYBR Green Dye A short summary describing SYBR green and how it is used to detect double-stranded DNA
Single-Drop Fluorimeter A PHOTO and description of the single-drop fluorimeter
Procedure
Smart Phone Camera Settings
[The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
Flash:
ISO setting:
White Balance:
Exposure:
Saturation:
Contrast:
[If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
Flash:
ISO setting:
White Balance:
Exposure:
Saturation:
Contrast:
Calibration
[Place a PHOTO of your camera phone set up in front of the flourimeter here]
Distance between the smart phone cradle and drop =
[See worksheet page 5]
Solutions Used for Calibration[See worksheet page 5]
row 1 cell 1
row 1 cell 2
row 1 cell 3
row 1 cell 4
row 2 cell 1
row 2 cell 2
row 2 cell 3
row 2 cell 4
row 2 cell 1
row 2 cell 2
row 2 cell 3
row 2 cell 4
DNA Sample Set-up Procedure
Step 1
Step 2
Step 3...
PCR Reaction Mix
What is in the PCR reaction mix?
DNA/ primer mix
What is in the DNA/ primer mix?
Research and Development
Specific Cancer Marker Detection - The Underlying Technology
(Add a write-up of the information discussed in Week 3's class)
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)