BME103 s2013:TEMPwu2

BME 103 Spring 2013	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: student Name: student Name: student Name: student Name: student Name: student LAB 2 WRITE-UP Background Information SYBR Green Dye A short summary describing SYBR green and how it is used to detect double-stranded DNA Single-Drop Fluorimeter A PHOTO and description of the single-drop fluorimeter Procedure Smart Phone Camera Settings [The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).] Flash: ISO setting: White Balance: Exposure: Saturation: Contrast: [If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).] Flash: ISO setting: White Balance: Exposure: Saturation: Contrast: Calibration [Place a PHOTO of your camera phone set up in front of the flourimeter here] Distance between the smart phone cradle and drop = [See worksheet page 5] Solutions Used for Calibration [See worksheet page 5] row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 DNA Sample Set-up Procedure Step 1 Step 2 Step 3... PCR Reaction Mix What is in the PCR reaction mix? DNA/ primer mix What is in the DNA/ primer mix? Research and Development Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)