BME103 s2013:TEMPwu2

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Data Analysis)
Current revision (18:05, 26 March 2013) (view source)
 
(10 intermediate revisions not shown.)
Line 21: Line 21:
| [[Image:BME103student.jpg|100px|thumb|Name: student]]
| [[Image:BME103student.jpg|100px|thumb|Name: student]]
|}
|}
 +
 +
<!-- Note: Delete any image placeholders that you do not need. -->
=LAB 2 WRITE-UP=
=LAB 2 WRITE-UP=
Line 28: Line 30:
'''SYBR Green Dye'''<br>
'''SYBR Green Dye'''<br>
-
''A short summary describing SYBR green and how it is used to detect double-stranded DNA''<br>
+
''[A short summary describing SYBR green dye]''<br>
'''Single-Drop Fluorimeter'''<br>
'''Single-Drop Fluorimeter'''<br>
-
''A PHOTO and description of the single-drop fluorimeter''<br>
+
''[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]''<br>
 +
'''How the Fluorescence Technique Works'''<br>
 +
''[In your own words, a summary of the information from page 9 of the worksheet]''
-
<br><br>
+
 
 +
<br>
 +
<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->
==Procedure==
==Procedure==
Line 60: Line 66:
'''Calibration'''<br>
'''Calibration'''<br>
-
''[Place a PHOTO that shows your camera phone set up in front of the flourimeter here]''
+
''[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]''
* Distance between the smart phone cradle and drop =
* Distance between the smart phone cradle and drop =
-
<br>''[See worksheet page 5]''
+
''[See worksheet page 5]''
Line 73: Line 79:
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
|-
|-
-
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4
+
| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4
|}
|}
 +
''[Add more rows as needed]''
 +
'''Placing Samples onto the Fluorimeter'''
 +
* ''[Step one, in your OWN words]''
 +
* ''[Step two, in your own words]''
 +
* ''[Step three, in your own words]''
 +
* ''[etc.]''
-
<br><br>
+
<br>
 +
<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->
==Data Analysis==
==Data Analysis==
-
'''Values From Image J''' ''[See worksheet page 5]''
+
'''Representative Images of Samples'''
 +
 
 +
''[Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample with no DNA]''
 +
 
 +
''[Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample '''with''' DNA (positive signal)]''
 +
 
 +
 
 +
 
 +
'''Image J Values for All Samples''' ''[See worksheet page 5]''
{| {{table}} width=700
{| {{table}} width=700
|-
|-
Line 91: Line 112:
| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4 || row 2 cell 5
| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4 || row 2 cell 5
|}
|}
-
 
''[Add more rows as needed]''
''[Add more rows as needed]''
-
'''Fitting a Straight Line'''
 
 +
'''Fitting a Straight Line'''<br>
 +
''[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]''
-
<br><br>
+
<br>
 +
<!-- Note: Be sure to delete the text in brackets: ''[ ]'' -->

Current revision

BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png


Contents

OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student


LAB 2 WRITE-UP

Background Information

SYBR Green Dye
[A short summary describing SYBR green dye]


Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]


How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]



Procedure

Smart Phone Camera Settings

  • [The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:
  • [If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
    • Flash:
    • ISO setting:
    • White Balance:
    • Exposure:
    • Saturation:
    • Contrast:


Calibration

[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]

  • Distance between the smart phone cradle and drop =

[See worksheet page 5]


Solutions Used for Calibration [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4

[Add more rows as needed]


Placing Samples onto the Fluorimeter

  • [Step one, in your OWN words]
  • [Step two, in your own words]
  • [Step three, in your own words]
  • [etc.]


Data Analysis

Representative Images of Samples

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5

[Add more rows as needed]


Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]




Personal tools