BME103 s2013:TEMPwu2: Difference between revisions
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| [[Image:BME103student.jpg|100px|thumb|Name: student]] | | [[Image:BME103student.jpg|100px|thumb|Name: student]] | ||
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=LAB 2 WRITE-UP= | =LAB 2 WRITE-UP= | ||
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'''SYBR Green Dye'''<br> | '''SYBR Green Dye'''<br> | ||
''A short summary describing SYBR green | ''[A short summary describing SYBR green dye]''<br> | ||
'''Single-Drop Fluorimeter'''<br> | '''Single-Drop Fluorimeter'''<br> | ||
''A | ''[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]''<br> | ||
'''How the Fluorescence Technique Works'''<br> | |||
''[In your own words, a summary of the information from page 9 of the worksheet]'' | |||
<br>< | <br> | ||
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==Procedure== | ==Procedure== | ||
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'''Calibration'''<br> | '''Calibration'''<br> | ||
''[ | ''[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]'' | ||
* Distance between the smart phone cradle and drop = | * Distance between the smart phone cradle and drop = | ||
''[See worksheet page 5]'' | |||
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| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4 | | row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4 | ||
|- | |- | ||
| row | | row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4 | ||
|} | |} | ||
''[Add more rows as needed]'' | |||
'''Placing Samples onto the Fluorimeter''' | |||
* ''[Step one, in your OWN words]'' | |||
* ''[Step two, in your own words]'' | |||
* ''[Step three, in your own words]'' | |||
* ''[etc.]'' | |||
<br>< | <br> | ||
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==Data Analysis== | ==Data Analysis== | ||
'''Representative Images of Samples''' | |||
''[Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample with no DNA]'' | |||
''[Show an IMAGE <u>where you drew a circle around the droplet</u> with the freehand tool for a sample '''with''' DNA (positive signal)]'' | |||
'''Image J Values for All Samples''' ''[See worksheet page 5]'' | |||
{| {{table}} width=700 | |||
|- | |||
| row 1 cell 1 || row 1 cell 2 || row 1 cell 3 || row 1 cell 4 || row 1 cell 5 | |||
|- | |||
| row 2 cell 1 || row 2 cell 2 || row 2 cell 3 || row 2 cell 4 || row 2 cell 5 | |||
|- | |||
| row 3 cell 1 || row 3 cell 2 || row 3 cell 3 || row 3 cell 4 || row 2 cell 5 | |||
|} | |||
''[Add more rows as needed]'' | |||
'''Fitting a Straight Line'''<br> | |||
''[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]'' | |||
<br>< | <br> | ||
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Latest revision as of 16:05, 26 March 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
[See worksheet page 5]
[Add more rows as needed]
Data AnalysisRepresentative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
[Add more rows as needed]
Fitting a Straight Line
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