BME103 s2013:TEMPwu2

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Protocols)
(Protocols)
Line 44: Line 44:
-
'''DNA Sample Set-up'''<br>
+
'''Calibration'''<br>
 +
 
 +
''[Place a PHOTO of your camera phone set up in front of the flourimeter here]''
 +
 
 +
* Distance between your smart phone cradle and drop =
{| {{table}}
{| {{table}}

Revision as of 03:29, 26 March 2013

BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png


Contents

OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student

LAB 2 WRITE-UP

Background Information

SYBR Green Dye
A short summary describing SYBR green and how it is used to detect double-stranded DNA


Single-Drop Fluorimeter
A PHOTO and description of the single-drop fluorimeter




Procedure

Smart Phone Camera Settings
The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).


Calibration

[Place a PHOTO of your camera phone set up in front of the flourimeter here]

  • Distance between your smart phone cradle and drop =
row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


PCR Reaction Mix

  • What is in the PCR reaction mix?


DNA/ primer mix

  • What is in the DNA/ primer mix?





Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)





Personal tools