BME103 s2013:TEMPwu2

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(OUR TEAM)
(Initial Machine Testing)
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=LAB 2 WRITE-UP=
=LAB 2 WRITE-UP=
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==Initial Machine Testing==
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==Background Information==
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'''The Original Design'''<br>
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'''SYBR Green Dye'''<br>
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(Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is)<br>
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''A short summary describing SYBR green and how it is used to detect double-stranded DNA''<br>
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'''Experimenting With the Connections'''<br>
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'''Single-Drop Fluorimeter'''<br>
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''A PHOTO and description of the single-drop fluorimeter''<br>
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When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)
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When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
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'''Test Run'''
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(Write the date you first tested Open PCR and your experience(s) with the machine)<br>
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Revision as of 03:20, 26 March 2013

BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png


Contents

OUR TEAM

Name: student
Name: student
Name: student
Name: student
Name: student
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Name: student
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Name: student
Name: student

LAB 2 WRITE-UP

Background Information

SYBR Green Dye
A short summary describing SYBR green and how it is used to detect double-stranded DNA


Single-Drop Fluorimeter
A PHOTO and description of the single-drop fluorimeter




Protocols

Thermal Cycler Program


DNA Sample Set-up

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4


DNA Sample Set-up Procedure

  1. Step 1
  2. Step 2
  3. Step 3...


PCR Reaction Mix

  • What is in the PCR reaction mix?


DNA/ primer mix

  • What is in the DNA/ primer mix?





Research and Development

Specific Cancer Marker Detection - The Underlying Technology

(Add a write-up of the information discussed in Week 3's class)

(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)





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