OUR TEAM
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LAB 2 WRITE-UP
Background Information
SYBR Green Dye
[A short summary describing SYBR green dye]
Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]
How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]
Procedure
Smart Phone Camera Settings
- Type of smart phone used and adjusted camera settings.
- Phone: HTC Droid Incredible 2
- Flash: None
- ISO setting: 800
- White Balance: Auto
- Exposure: +2
- Saturation: +2
- Contrast:-2
Calibration
[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
- Distance between the smart phone cradle and drop = 3.7 cm
[See worksheet page 5]
Solutions Used for Calibration [See worksheet page 5]
Image J Values for All Samples [See worksheet page 5]
Calf Thymus DNA solution concentration (microg/mL) |
Volume of the 2X DNA solution (µL) |
Volume of the SYBR GREEN I Dye solution (µL) |
Final DNA concentration in PicoGreen Assay (ng/mL)
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5 |
80 |
80 |
2.5
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2 |
80 |
80 |
1
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1 |
80 |
80 |
0.5
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0.5 |
80 |
80 |
0.25
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0.25 |
80 |
80 |
0.125
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0 |
80 |
80 |
blank
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[Add more rows as needed]
Placing Samples onto the Fluorimeter
- [Step one, in your OWN words]
- [Step two, in your own words]
- [Step three, in your own words]
- [etc.]
Data Analysis
Representative Images of Samples
Sample with no DNA
Sample with DNA (positive signal)
Calf Thymus DNA concentration (FINAL), migrog/mL |
Area |
Mean Pixel Value |
RAWINTDEN of the drop |
RAWINTDEN of the background
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Water |
40636 |
32.437 |
1318101 |
302462
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0.25 |
39770 |
64.565 |
2567761 |
243302
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0.25 |
43440 |
48.047 |
2087153 |
50662
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0.25 |
38994 |
82.158 |
3203667 |
239145
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0.5 |
45724 |
105.339 |
4770813 |
286980
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0.5 |
37690 |
83.846 |
3160168 |
251141
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0.5 |
38724 |
73.079 |
2829899 |
54829
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1.00 |
41200 |
105.656 |
4353036 |
288678
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1.00 |
43584 |
113.023 |
4926005 |
335726
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1.00 |
41404 |
105.037 |
4348994 |
279375
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2.00 |
40024 |
135.205 |
5411434 |
326434
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2.00 |
40968 |
103.250 |
4229944 |
260408
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2.00 |
40111 |
129.871 |
5209254 |
425061
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5.00 |
40950 |
132.782 |
5437418 |
393246
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5.00 |
40468 |
147.876 |
5984250 |
352233
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5.00 |
39796 |
140.267 |
5582067 |
341520
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Fitting a Straight Line
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