BME103 s2013:T900 Group9 L2

BME 103 Spring 2013	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Coley White Name: Aimen Vanood Name: student Name: student LAB 2 WRITE-UP Background Information SYBR Green Dye [A short summary describing SYBR green dye] Single-Drop Fluorimeter [A description of the single-drop fluorimeter device. Add a PHOTO for bonus points] How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet] Procedure Smart Phone Camera Settings Type of smart phone used and adjusted camera settings. Phone: HTC Droid Incredible 2 Flash: None ISO setting: 800 White Balance: Auto Exposure: +2 Saturation: +2 Contrast:-2 Calibration [Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.] Distance between the smart phone cradle and drop = 3.7 cm [See worksheet page 5] Solutions Used for Calibration [See worksheet page 5] Image J Values for All Samples [See worksheet page 5] Calf Thymus DNA solution concentration (microg/mL) Volume of the 2X DNA solution (µL) Volume of the SYBR GREEN I Dye solution (µL) Final DNA concentration in PicoGreen Assay (ng/mL) 5 80 80 2.5 2 80 80 1 1 80 80 0.5 0.5 80 80 0.25 0.25 80 80 0.125 0 80 80 blank Placing Samples onto the Fluorimeter [Step one, in your OWN words] [Step two, in your own words] [Step three, in your own words] [etc.] Data Analysis Representative Images of Samples Sample with no DNA Sample with DNA (positive signal) Calf Thymus DNA concentration (FINAL), migrog/mL Area Mean Pixel Value RAWINTDEN of the drop RAWINTDEN of the background Water 40636 32.437 1318101 302462 0.25 39770 64.565 2567761 243302 0.25 43440 48.047 2087153 50662 0.25 38994 82.158 3203667 239145 0.5 45724 105.339 4770813 286980 0.5 37690 83.846 3160168 251141 0.5 38724 73.079 2829899 54829 1.00 41200 105.656 4353036 288678 1.00 43584 113.023 4926005 335726 1.00 41404 105.037 4348994 279375 2.00 40024 135.205 5411434 326434 2.00 40968 103.250 4229944 260408 2.00 40111 129.871 5209254 425061 5.00 40950 132.782 5437418 393246 5.00 40468 147.876 5984250 352233 5.00 39796 140.267 5582067 341520 Fitting a Straight Line