OUR TEAM
Name: Anthony Zingale Role: Experimental Protocol Planner
Name: Josh Snyder Role: Machine Tester
Name: Adam Pak Role:Experimental Protocol Planner
Name: Sunhine Silvas Role: Machine Tester
Name: Renee Tran Role:Research and Development Scientist
LAB 2 WRITE-UP
Background Information
SYBR Green Dye
molecluar diagram of SYBR Green I
SYBR Green I is a dye that is used as a nucleic acid stain in several fields such as biochemistry, and molecualr biology. The way that this compund works is by absorbing the blue light and emits green light
Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]
How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]
Procedure
Smart Phone Camera Tech Specs
Phone :
iphone 4
Tech specs
" 5-megapixel iSight camera
Video recording, HD (720p) up to 30 frames per second with audio
VGA-quality photos and video at up to 30 frames per second with the front camera
Tap to focus video or still images
LED flash
Photo and video geotagging "
Source of Tech specs
http://www.apple.com/iphone/iphone-4/specs.html
Smart Phone Camera Settings
Settings :
Settings used in lab
Observations
Flash:
Turned off
N/A
ISO setting:
ISO setting 80-1000
No control over ISO settings
White Balance:
Auto White balance
No control over white balance
Exposure:
Auto exposure
No control over exposure
Saturation:
Very high
No control over saturation
Contrast:
Very high
No control over contrast
Source:
http://www.imaging-resource.com/PRODS/IPHONE4/IPHONE4A.HTM
This source was used to find out what were the defult settings of iphone 4
No additional phones or cameras used
Calibration
Diagram of the set up
Distance and angles between the smart phone and =
candle = 3.62cm
table = 5.67cm
angle from camera to drop = approx 90°
Solutions Used for Calibration
Solution
Concetration of SYBR green
1
None (Water)
2
0.25 micrograms per ml
3
0.05 micrograms per ml
4
1.0 micrograms per ml
5
2.0 micrograms per ml
6
5.0 micrograms per ml
Placing Samples onto the Fluorimeter Procedures
Aquire following materials :
Materials :
Set of read SYBR green(5 samples)
Water
pipette
Superhydrophobic surface
cradle
Smart phone
Box
plastic base
small box to set up the phone
Calibrate phone
Carefully palce one drop of sample onto Superhydrophobic surface
Place Superhydrophobic surface onto cradle
Place the big box over cradle to create a dark room
Set up smart phone using plastic base
Take a picture
Repeat steps 2-6 for all 6 samples
Measure distances of phone to table and to cradle
Analyze pictures and write down the observations
Data Analysis
Representative Images of Samples
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
row 1 cell 1
row 1 cell 2
row 1 cell 3
row 1 cell 4
row 1 cell 5
row 2 cell 1
row 2 cell 2
row 2 cell 3
row 2 cell 4
row 2 cell 5
row 3 cell 1
row 3 cell 2
row 3 cell 3
row 3 cell 4
row 2 cell 5
[Add more rows as needed]
Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]