OUR TEAM
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LAB 2 WRITE-UP
Background Information
SYBR Green Dye
[A short summary describing SYBR green dye]
Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]
How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]
Procedure
Smart Phone Camera Tech Specs
Phone : |
iphone 4
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Tech specs |
" 5-megapixel iSight camera
Video recording, HD (720p) up to 30 frames per second with audio
VGA-quality photos and video at up to 30 frames per second with the front camera
Tap to focus video or still images
LED flash
Photo and video geotagging "
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Source of Tech specs |
http://www.apple.com/iphone/iphone-4/specs.html
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Smart Phone Camera Settings
Settings : |
Settings used in lab |
Observations
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Flash: |
Turned off |
N/A
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ISO setting: |
ISO setting 80-1000 |
No control over ISO settings
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White Balance: |
Auto White balance |
No control over white balance
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Exposure: |
Auto exposure |
No control over exposure
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Saturation: |
Very high |
No control over saturation
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Contrast: |
Very high |
No control over contrast
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Source: |
http://www.imaging-resource.com/PRODS/IPHONE4/IPHONE4A.HTM |
This source was used to find out what were the defult settings of iphone 4
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- No additional phones or cameras used
Calibration
Distance and angles between the smart phone and =
- candle = 3.62cm
- table = 5.67cm
- angle from camera to drop = approx 90°
Solutions Used for Calibration
Solution |
Concetration of SYBR green
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1 |
None (Water)
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2 |
0.25 micrograms per ml
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3 |
0.05 micrograms per ml
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4 |
1.0 micrograms per ml
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5 |
2.0 micrograms per ml
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6 |
5.0 micrograms per ml
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Placing Samples onto the Fluorimeter Procedures
- Aquire following materials :
Materials :
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Set of read SYBR green(5 samples)
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Water
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pipette
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Superhydrophobic surface
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cradle
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Smart phone
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Box
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plastic base
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small box to set up the phone
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- Calibrate phone
- Carefully palce one drop of sample onto Superhydrophobic surface
- Place Superhydrophobic surface onto cradle
- Place the big box over cradle to create a dark room
- Set up smart phone using plastic base
- Take a picture
- Repeat steps 2-6 for all 6 samples
- Measure distances of phone to table and to cradle
- Analyze pictures and write down the observations
Data Analysis
Representative Images of Samples
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
row 1 cell 1 |
row 1 cell 2 |
row 1 cell 3 |
row 1 cell 4 |
row 1 cell 5
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row 2 cell 1 |
row 2 cell 2 |
row 2 cell 3 |
row 2 cell 4 |
row 2 cell 5
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row 3 cell 1 |
row 3 cell 2 |
row 3 cell 3 |
row 3 cell 4 |
row 2 cell 5
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[Add more rows as needed]
Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]
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