BME103 s2013:T900 Group7 L2: Difference between revisions
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'''Placing Samples onto the Fluorimeter''' | '''Placing Samples onto the Fluorimeter''' | ||
# | # Align the slide with the blue LED, so that the light passes in the middle of two rows of wells and reaches the middle of the opposite side. | ||
# | # Place 80 μL of SYBR GREEN in-between two wells. | ||
# | # Next, add 80 μL of DNA sample in the drop that is formed from step #2. | ||
# | # Cover this created sample from light in order to visualize the florescence. | ||
# Take a picture of the sample. | |||
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Revision as of 21:06, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye SAMANTHA
How the Fluorescence Technique Works SAMANTHA
ProcedureSmart Phone Camera Settings
Calibration The distance between the smart phone cradle and the drop is approximately 10 cm.
Solutions Used for Calibration
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Samples
Image J Values for All Samples
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