BME103 s2013:T900 Group7 L2: Difference between revisions
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| Calf Thymus DNA solution concentration (micro/mL) || Volume of the 2X DNA solution (μL) || Volume of the SYBR GREEN I Dye solution (μL) || Final DNA concentration in PicoGreen Assay (ng/mL) | | Calf Thymus DNA solution concentration (micro/mL) || Volume of the 2X DNA solution (μL) || Volume of the SYBR GREEN I Dye solution (μL) || Final DNA concentration in PicoGreen Assay (ng/mL) | ||
|- | |- | ||
| | | 5 || 80 || 80 || 2.5 | ||
|- | |- | ||
| | | 2 || 80 || 80 || 1 | ||
|- | |- | ||
| | | 1 || 80 || 80 || 0.5 | ||
|- | |- | ||
| | | 0.5 || 80 || 80 || 0.25 | ||
|- | |- | ||
| | | 0.25 || 80 || 80 || 0.125 | ||
|- | |- | ||
| | | 0 || 80 || 80 || blank | ||
|} | |} | ||
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Revision as of 21:11, 31 March 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye SAMANTHA
ProcedureSmart Phone Camera Settings
Calibration AMANDA [Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
Solutions Used for Calibration AMANDA [See worksheet page 5]
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Data AnalysisRepresentative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
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Fitting a Straight Line
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