OUR TEAM
|
|
|
|
|
|
LAB 2 WRITE-UP
Background Information
SYBR Green Dye
[A short summary describing SYBR green dye]
Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]
How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]
Procedure
Smart Phone Camera Settings
- Iphone 5
- Flash: None
- ISO setting: NA
- White Balance: NA
- Exposure: NA
- Saturation: NA
- Contrast: NA
Calibration
Photo of Fluorimeter
- Distance between the smart phone cradle and drop = 8cm
Solutions Used for Calibration
Calf Thymus DNA solution concentration (microg/mL) |
Volume of the 2X DNA solution (uL) |
Volume of the SYBR GREEN I Dye Solution (uL) |
Final DNA concentration in PicoGreen Assay (ng/mL)
|
0 |
80 |
80 |
blank
|
.25 |
80 |
80 |
.125
|
.5 |
80 |
80 |
.25
|
1 |
80 |
80 |
.5
|
2 |
80 |
80 |
1
|
5 |
80 |
80 |
2.5
|
Placing Samples onto the Fluorimeter
- Get Slide, Micropipette and vial of sample
- Place slide into fluorimeter
- Take 80 micro liters of sample into micropipette
- Place 4 drops onto the slide, slide the slide over to where the light hits, don't use the same place twice (place new drops in different area)
- Make sure the drops form a ball and don't spread around individually
- Clean Up Workstation
Data Analysis
Representative Images of Samples
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
row 1 cell 1 |
row 1 cell 2 |
row 1 cell 3 |
row 1 cell 4 |
row 1 cell 5
|
row 2 cell 1 |
row 2 cell 2 |
row 2 cell 3 |
row 2 cell 4 |
row 2 cell 5
|
row 3 cell 1 |
row 3 cell 2 |
row 3 cell 3 |
row 3 cell 4 |
row 2 cell 5
|
[Add more rows as needed]
Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]
|