BME103 s2013:T900 Group6 L2: Difference between revisions
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* Take 80 micro-liters of SYBR Green solution into micropipette | * Take 80 micro-liters of SYBR Green solution into micropipette | ||
* Release all of the 80 micro-liters of SYBR Green fluid onto the slide, then ensure that the beam of light hits the droplet. | * Release all of the 80 micro-liters of SYBR Green fluid onto the slide, then ensure that the beam of light hits the droplet. | ||
* | *With a new plastic micropipette, take another 80 micro-liters of DNA Sample and extract into the SYBR Green droplet. Make sure the drops form a ball and doesn't spread around making individual droplets all over the slide. | ||
*After the photo shoot is complete, calibrate the micropipette to 160 micro-liters and extract all of the sample on the slide. | *In order to see the glow of the SYBR Green with the DNA sample solution, a dark environment must be in place to see the dim green light. Place a box casing over the machine and take multiple pictures of the glowing solution. | ||
*After the photo shoot is complete, and pictures are saved onto the phone, calibrate the micropipette to 160 micro-liters and extract all of the sample on the slide. | |||
*Repeat the same steps for the additional DNA Samples. | *Repeat the same steps for the additional DNA Samples. | ||
* Clean Up Workstation | * Clean Up Workstation |
Revision as of 21:57, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
Calibration Photo of Fluorimeter
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
[Add more rows as needed]
Fitting a Straight Line
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