BME103 s2013:T900 Group6 L2: Difference between revisions
Cyril Wassef (talk | contribs) |
Cyril Wassef (talk | contribs) |
||
Line 30: | Line 30: | ||
'''SYBR Green Dye'''<br> | '''SYBR Green Dye'''<br> | ||
''[ | ''[SBYR green dye is a nucleic acid-sensitive gel dye that is useful in pinpointing double stranded DNA in samples within open PCR machines and other utilities such as the single-drop fluorimeter.]''<br> | ||
'''Single-Drop Fluorimeter'''<br> | '''Single-Drop Fluorimeter'''<br> | ||
''[ | ''[The single-drop fluorimeter is a fluorescence-based DNA detection device that aids in bringing to light PCR-Amplified DNA. The DNA sample is placed on a plate placed upon the machine and shined with a blue LED light. When shone upon, the sample's DNA begins to emit a green light; able to be captured with a cell phone camera.]''<br> | ||
Revision as of 19:50, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
Calibration Photo of Fluorimeter
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
[Add more rows as needed]
Fitting a Straight Line
|