BME103 s2013:T900 Group5 L2: Difference between revisions
(18 intermediate revisions by 4 users not shown) | |||
Line 17: | Line 17: | ||
| [[Image:BME103student.jpg|100px|thumb|Name: Matt McClintock]] | | [[Image:BME103student.jpg|100px|thumb|Name: Matt McClintock]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: Heewon Park]] | | [[Image:BME103student.jpg|100px|thumb|Name: Heewon Park]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: Jasmine Joseph]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: | | [[Image:BME103student.jpg|100px|thumb|Name: Cody Gates]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: student]] | | [[Image:BME103student.jpg|100px|thumb|Name: student]] | ||
|} | |} | ||
Cody Gates made 20% contribution to the lab although he may not be noted in the edit history. | |||
<!-- Note: Delete any image placeholders that you do not need. --> | <!-- Note: Delete any image placeholders that you do not need. --> | ||
Line 33: | Line 34: | ||
'''Single-Drop Fluorimeter'''<br> | '''Single-Drop Fluorimeter'''<br>[[Image:Fluorimeter.jpg]]<Br> | ||
A single-drop fluorimeter is a type of device that is used to measure patterns of fluorescence in relation to the number of molecules within a medium.<br> | A single-drop fluorimeter is a type of device that is used to measure patterns of fluorescence in relation to the number of molecules within a medium.<br> | ||
Line 47: | Line 48: | ||
'''Smart Phone Camera Settings'''<br> | '''Smart Phone Camera Settings'''<br> | ||
* ''[ | * ''[iphone 4]'' | ||
** Flash: Off | ** Flash: Off | ||
** ISO setting: Auto | ** ISO setting: Auto | ||
Line 54: | Line 55: | ||
** Saturation: Auto | ** Saturation: Auto | ||
** Contrast: Auto | ** Contrast: Auto | ||
Line 71: | Line 64: | ||
* Distance between the smart phone cradle and drop = | * Distance between the smart phone cradle and drop = 6.5 cm | ||
Line 97: | Line 89: | ||
* 2.) 80 μL of 5μg/mL of calf thymus concentration DNA samples were then added to the slide <br> | * 2.) 80 μL of 5μg/mL of calf thymus concentration DNA samples were then added to the slide <br> | ||
* 3.) To take 3 pictures in the the dark with as much of the blue LED light as possible, we used a timer on the camera, set to 3 seconds. <br> | * 3.) To take 3 pictures in the the dark with as much of the blue LED light as possible, we used a timer on the camera, set to 3 seconds. <br> | ||
* 4.) The slide, now containing | * 4.) The slide, now containing SYBR Green I and Calf Thymus DNA was then aligned in the cradle such that the light was shining into the DNA. <br> | ||
* 5.) With the Camera phone, we captured 3 images of the DNA and removed the light box. <br> | * 5.) With the Camera phone, we captured 3 images of the DNA and removed the light box. <br> | ||
* 6.) That sample was then disposed of with a micropipette <br> | * 6.) That sample was then disposed of with a micropipette <br> | ||
* 7.) Repeat with 80μL Green | * 7.) Repeat with 80μL Green SYBR I and 80μL of each Calf Thymus Concentration given by the first column in the table above<br> | ||
==Data Analysis== | ==Data Analysis== | ||
Line 106: | Line 98: | ||
'''Representative Images of Samples''' | '''Representative Images of Samples''' | ||
'' | '' no DNA ''<Br> | ||
[[Image:NoDNA.JPG]] | |||
''with DNA''(positive signal)]''<Br> | |||
[[Image:DNA10.JPG]] | |||
<br> | |||
<br> | |||
[[Image:Excel.jpg]] | |||
<br> | |||
''flotting and fitting a linear graph'' | |||
<br> | |||
[[Image:Bme103-2.jpg]] | |||
<br><font color=red>**PLAGIARISM WARNING: A DUPLICATE VERSION OF THIS IMAGE HAS BEEN FOUND ON ANOTHER TEAM'S LAB REPORT**</font> | |||
Latest revision as of 09:10, 28 June 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||
OUR TEAMCody Gates made 20% contribution to the lab although he may not be noted in the edit history.
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
Data AnalysisRepresentative Images of Samples
|