BME103 s2013:T900 Group5 L2: Difference between revisions
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darken the area. The Smart phone was aligned with the slide to get a sideways picture of the DNA sample. This creates the ideal angle to observe any | darken the area. The Smart phone was aligned with the slide to get a sideways picture of the DNA sample. This creates the ideal angle to observe any | ||
fluorescent light after the picture is taken. The camera phone is calibrated to view the fluorescent light with little interference from contrast and | fluorescent light after the picture is taken. The camera phone is calibrated to view the fluorescent light with little interference from contrast and | ||
recording the most light- done by setting the exposure high. The saturation is set to low so that color is not rendered in the photo. | recording the most light- done by setting the exposure high. The saturation is set to low so that color is not rendered in the photo. The ISO is set to high to maximize focus in the dark setting. With these settings, the fluorescence in the sample is easily observed. | ||
Revision as of 22:58, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
ProcedureSmart Phone Camera Settings
[See worksheet page 5]
Data AnalysisRepresentative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5] [Add more rows as needed]Fitting a Straight Line[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]
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