SYBR Green Dye [A short summary describing SYBR green dye]
Single-Drop Fluorimeter [A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]
How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet]
Procedure
Smart Phone Camera Settings
[The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
Flash:
ISO setting:
White Balance:
Exposure:
Saturation:
Contrast:
[If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
Flash:
ISO setting:
White Balance:
Exposure:
Saturation:
Contrast:
Calibration
[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
Distance between the smart phone cradle and drop =
[See worksheet page 5]
Solutions Used for Calibration[See worksheet page 5]
Concentration Calf Thymus (μg/mL)
Volume DNA added (μL)
Volume SABR GREEN I (μL)
Concentration resulting DNA ng/mL
5
80
80
2.5
2
80
80
1
1
80
80
0.5
0.5
80
80
0.25
0.25
80
80
.125
0
80
80
blank
Placing Samples onto the Fluorimeter
Step 1.) With the Camera set in place we prepared the slide with SYBR Green I, a constant throughout the experiment
Step 2.) 80 μL of 5μg/mL of calf thymus concentration DNA samples were then added to the slide
Step 3.) To take 3 pictures in the the dark with as much of the blue LED light as possible, we used a timer on the camera, set to 3 seconds.
Step 4.) The slide, now containing SYBER Green I and Calf Thymus DNA was then aligned in the cradle such that the light was shining into the DNA.
Step 5.) With the Camera phone, we captured 3 images of the DNA and removed the light box.
Step 6.) That sample was then disposed of with a micropipette
Step 7.) Repeat with 80μL Green SABR I and 80μL of each Calf Thymus Concentration given by the first column in the table above
Data Analysis
Representative Images of Samples
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples[See worksheet page 5]
[Add more rows as needed]Fitting a Straight Line [Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]