BME103 s2013:T900 Group4 L2: Difference between revisions
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'''Smart Phone Camera Settings'''<br> | '''Smart Phone Camera Settings'''<br> | ||
* '' | * ''Iphone 4'' | ||
** Flash: Off | ** Flash: Off | ||
** ISO setting:NA | ** ISO setting:NA | ||
Line 92: | Line 92: | ||
'''Placing Samples onto the Fluorimeter''' | '''Placing Samples onto the Fluorimeter''' | ||
* Step one, | * Step one, Check your phone camera setting, and turn the flash off | ||
* Step two,Pick up DAN, gloves, lab coat, glass slide, buffer tubes, sync green I tube, and DNA standard | * Step two,Pick up DAN, gloves, lab coat, glass slide, buffer tubes, sync green I tube, and DNA standard | ||
* Step three, Slide the glass slide into fluorimeter | * Step three, Slide the glass slide into fluorimeter | ||
* Step four, Drop 80 micro liters of sample of the solution where the light is flashing | * Step four, Drop 80 micro liters of sample of the solution where the light is flashing | ||
* Step five, | * Step five, Put the box around it and take pictures of the drops | ||
==Data Analysis== | ==Data Analysis== |
Revision as of 18:02, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye SYBR Green I is a fluorescent dye that is effectively used to bind double stranded DNA. Single-Drop Fluorimeter Fluorimeter is a device that is used to measure fluorescence. How the Fluorescence Technique Works
ProcedureSmart Phone Camera Settings
Data AnalysisRepresentative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
[Add more rows as needed]
Fitting a Straight Line
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