BME103 s2013:T900 Group4 L2: Difference between revisions
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'''Calibration''' Photo of Fluorimeter | '''Calibration''' Photo of Fluorimeter | ||
* | * Put your phone on the cradle at a right angle | ||
* | * Then fix the height of the fluorimeter so that the camera can take picture of the drop on sideways | ||
[[Image:Group4_Fluorometer.JPG]] | [[Image:Group4_Fluorometer.JPG]] |
Revision as of 15:13, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye SYBR Green I (SG) is an asymmetrical cyanine dye used as a nucleic acid stain in molecular biology. SYBR Green I binds to DNA. The resulting DNA-dye-complex absorbs blue light (λmax = 497 nm) and emits green light (λmax = 520 nm). The stain preferentially binds to double-stranded DNA, but will stain single-stranded DNA with lower performance. SYBR green can also stain RNA with a lower performance than DNA. SYBR green finds usage in several areas of biochemistry and molecular biology. It is used as a dye for the quantification of double stranded DNA in some methods of real time PCR. It is also used to visualize DNA in gel electrophoresis. Higher concentrations of SYBR green can be used to stain agarose gels in order to visualize the DNA present. In addition to labeling pure nucleic acids SYBR green can also be used for labeling of DNA within cells for flow cytometry and fluorescence microscopy. In these cases RNase treatment may be required to reduce background from RNA in the cells. http://en.wikipedia.org/wiki/SYBR_Green_I Single-Drop Fluorimeter Fluorimeter is a device that is used to measure fluorescence. How the Fluorescence Technique Works
ProcedureSmart Phone Camera Settings
Data AnalysisRepresentative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
[Add more rows as needed]
Fitting a Straight Line
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