BME103 s2013:T900 Group4 L2

BME 103 Spring 2013	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Kinjal Ahir : Procedure Name: student Name: student Name: student Name: student Name: student LAB 2 WRITE-UP Background Information SYBR Green Dye [A short summary describing SYBR green dye] Single-Drop Fluorimeter [A description of the single-drop fluorimeter device. Add a PHOTO for bonus points] How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet] Procedure Smart Phone Camera Settings [Iphone 4] Flash: NA ISO setting:NA White Balance: NA Exposure: NA Saturation: NA Contrast: NA Calibration<Photo of Fluorimeter> [Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.] Distance between the smart phone cradle and drop = [8cm] Solutions Used for Calibration [See worksheet page 5] Calf Thymus DNA solution concentration (microg/ML) Volume of the 2x DNA solution (uL) Volume of the SYBR GREEN Dye Solution (uL) Final DNA concentration PicoGreen Assay (ng/mL) 0 80 80 .25 80 80 row 3 cell 4 .5 80 80 row 4 cell 4 1 80 80 row 5 cell 4 2 80 80 row 6 cell 4 5 80 80 row 7 cell 4 Placing Samples onto the Fluorimeter [Step one, in your OWN words] [Step two, in your own words] [Step three, in your own words] [etc.] Data Analysis Representative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)] Image J Values for All Samples [See worksheet page 5] Trial DNA Concentration (μg/mL) Area Mean Pixel Value RAWINTDEN (drop) RAWINTDEN (background) 1 0 10,906 21.672 236,351 70,338 1 0.25 10,044 35.971 361,290 42,572 1 0.5 10,562 84.274 890,103 52,974 1 0.5 10,452 50.546 528,303 50,012 1 1 10,350 115.226 1,192,594 39,995 1 2 10,914 193.145 2,107,989 46,923 1 5 10,282 142.563 1,465,554 55,342 2 0 10,808 50.412 544,856 71,106 2 0.25 10,510 51.038 536,412 39,795 2 0.5 10,688 78.799 842,203 57,075 2 1 10,432 90.965 948,944 52,390 2 2 10,103 152.075 1,536,481 61,000 2 5 10,440 115.674 1,207,635 51,873 3 0 10,131 37.264 377,520 66,289 3 0.25 10,448 61.987 647,645 48,887 3 0.5 10,762 64.475 693,875 40,978 3 1 10,465 74.835 783,144 47,549 3 2 10,188 155.63 1,585,554 43,186 3 5 10,672 72.312 771,711 47,142 [Add more rows as needed] Fitting a Straight Line [Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]