BME103 s2013:T900 Group4 L2: Difference between revisions
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{| {{table}} width=700 | {| {{table}} width=700 | ||
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| Calf Thymus DNA | | Calf Thymus DNA Solution Concentration (μg/mL) || Volume of the 2x DNA Solution (μL) || Volume of the SYBR GREEN I Dye Solution (μL) || Final DNA Concentration PicoGreen Assay (ng/mL) | ||
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| 5 || 80 || 80 || 2.5 | | 5 || 80 || 80 || 2.5 | ||
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'''Placing Samples onto the Fluorimeter''' | '''Placing Samples onto the Fluorimeter''' | ||
* Step one: | * Step one: Adjust camera settings and turn the flash off | ||
* Step two: Obtain calf thymus DNA, gloves, lab coat, glass slide, buffer tubes, and SYBR Green I | * Step two: Obtain calf thymus DNA, gloves, lab coat, glass slide, buffer tubes, and SYBR Green I | ||
* Step three: | * Step three: Insert Teflon-coated glass slide into fluorimeter | ||
* Step four: Drop 80μL of solution onto slide in front of blue light | * Step four: Drop 80μL of solution onto slide and position in front of blue light | ||
* Step five: Put dark box over fluorimeter and smartphone and take pictures of the drops | * Step five: Put dark box over fluorimeter and smartphone and take pictures of the drops | ||
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As shown, the DNA positive drop is much brighter. | As shown, the DNA positive drop is much brighter than the DNA negative drop. | ||
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'''Fitting a Straight Line'''<br> | |||
[[Image:LineFit.jpg|700px]] | |||
'' | |||
''General trendline of INTDENS readings vs. DNA concentration'' | |||
Latest revision as of 09:04, 2 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye Single-Drop Fluorimeter
How the Fluorescence Technique Works
ProcedureSmart Phone Camera Settings
Data AnalysisRepresentative Images of Samples
General trendline of INTDENS readings vs. DNA concentration
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