BME103 s2013:T900 Group4 L2: Difference between revisions
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'''SYBR Green Dye'''<br> | '''SYBR Green Dye'''<br> | ||
SYBR Green I is a fluorescent dye that is effectively used to bind double stranded DNA. This dye then emits a green light. SYBR green dye is commonly used because it is so efficient at visualizing DNA and is incredibly safe to users. Not to mention large quantities of the dye are cheap, making it a prime dye for basic DNA displaying. | SYBR Green I is a fluorescent dye that is effectively used to bind double stranded DNA. This dye then emits a green light. SYBR green dye is commonly used because it is so efficient at visualizing DNA and is incredibly safe to users. Not to mention large quantities of the dye are cheap, making it a prime dye for basic DNA displaying. | ||
'''Single-Drop Fluorimeter'''<br> | '''Single-Drop Fluorimeter'''<br> | ||
Fluorimeter is a device that is used to measure parameters of fluorescence concentration. The device usually measures fixed wavelengths either by filters, monochromators or fixed diodes. The fluorometer have two light detectors. One light detects the absorbance, while the other detects fluorescence emission. | Fluorimeter is a device that is used to measure parameters of fluorescence concentration. The device usually measures fixed wavelengths either by filters, monochromators or fixed diodes. The fluorometer have two light detectors. One light detects the absorbance, while the other detects fluorescence emission. | ||
'''How the Fluorescence Technique Works'''<br> | '''How the Fluorescence Technique Works'''<br> | ||
Fluorescence Technique is a technique to measure concentration of mixing liquids. This is done by using a single drop fluorometer that contain a slide coated with a layer of Teflon. The slide also contain circles of bare glass, allowing drops of liquids to stay in place. Blue LED light from the fluorometer points to the drops and increase intensity of the fluorescence. SYBR Green I was later added with a solvent to help form the complex DNA. | Fluorescence Technique is a technique to measure concentration of mixing liquids. This is done by using a single drop fluorometer that contain a slide coated with a layer of Teflon. The slide also contain circles of bare glass, allowing drops of liquids to stay in place. Blue LED light from the fluorometer points to the drops and increase intensity of the fluorescence. SYBR Green I was later added with a solvent to help form the complex DNA. |
Revision as of 22:33, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
Single-Drop Fluorimeter
How the Fluorescence Technique Works
ProcedureSmart Phone Camera Settings
Photo of Fluorimeter
Data AnalysisRepresentative Images of Samples Circle analysis of DNA negative drop. 0 concentration. Circle Analysis of DNA positive drop. 5 concentration.
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Fitting a Straight Line
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