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The Original Design
(Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is)
Experimenting With the Connections
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer)
When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
Test Run
(Write the date you first tested Open PCR and your experience(s) with the machine)
Protocols
Thermal Cycler Program
DNA Sample, 50 μL each: patient ID's from the UnderGrad Teaching Assistant(UGTA).
Positive control
Patient 1
Patient 1
Patient1
cancer DNA template
ID____________
ID____________
ID_____________
Replicate 1
Replicate 2
Replicate 3
Tube label_______
Tube label_______
Tube label_______
Tube label_______
Negative Control: no
Patient 2
Patient 2
Patient 2
DNA template
ID_________
ID____________
ID____________
Replicate 1
Replicate 2
Replicate 3
Tube label_______
Tube label_______
Tube label_______
Tube label_______
DNA Sample Set-up Procedure
Step 1: Student were Provided with 8 tubes of 50 μL PCR reaction mix, 8 tubes of 50 μL diluted template + primers, and disposable transfer pipettes.
Step 2: The instructors were provided to test positive and negative for a DNA marker.
Step 3: Protocol planner set up the DNA and ran the PCR reactions.
PCR Reaction Mix
In the PCR reaction mix there is Mix contains Taq DNA polymerase, MgCl2, dNTP's, forward primer, reverse primer.
DNA/ primer mix
What is in the DNA/ primer mix?
Research and Development
Specific Cancer Marker Detection - The Underlying Technology
(Add a write-up of the information discussed in Week 3's class)
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)