BME103 s2013:T900 Group1 L2: Difference between revisions
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| [[Image:BME103 Group1 KID.jpeg|100px|thumb|Kristi Norris:<br>Protocol/Procedure]] | | [[Image:BME103 Group1 KID.jpeg|100px|thumb|Kristi Norris:<br>Protocol/Procedure]] | ||
| [[Image:BME103_Group1_Carlos2.jpg|100px|thumb|Carlos Renteria:<br>Research and Design Specialist]] | | [[Image:BME103_Group1_Carlos2.jpg|100px|thumb|Carlos Renteria:<br>Research and Design Specialist]] | ||
| [[Image: | | [[Image:Guitar.jpg|100px|thumb|Raul Monzolo:<br>Open PCR Machine Engineer]] | ||
| [[Image:BME103 Group1 Johnny.jpg|100px|thumb|Johnny Montez:<br>Open PCR Machine Engineer]] | | [[Image:BME103 Group1 Johnny.jpg|100px|thumb|Johnny Montez:<br>Open PCR Machine Engineer]] | ||
| [[Image: | | [[Image:Elephant1.jpg|100px|thumb|Robert Sanchez:<br>Research and Design Specialist]] | ||
| [[Image:BME103_Group1.JPG|300px|thumb|Group 1]] | | [[Image:BME103_Group1.JPG|300px|thumb|Group 1]] | ||
|} | |} | ||
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'''SYBR Green Dye'''<br> | '''SYBR Green Dye'''<br> | ||
SYBR Green | SYBR Green Dye is a detection probe that absorbs blue wavelengths of light and emits green wavelengths, enabling it to bind to double-stranded DNA. Although it performs well in the presence of double-stranded DNA, it fluoresces less effectively in water and with single-stranded DNA. | ||
[[Image:SYBRgreen.jpg]] | |||
'''Single-Drop Fluorimeter'''<br> | '''Single-Drop Fluorimeter'''<br> | ||
Fluorimeter is a device that is | A Fluorimeter is a device used to quantitatively measure fluorescent material in a sample or substance in such a way that it is proportional to the amount of molecules being detected. | ||
[[Image:Photo(2).JPG]] | [[Image:Photo(2).JPG]] | ||
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'''How the Fluorescence Technique Works'''<br> | '''How the Fluorescence Technique Works'''<br> | ||
The components of a fluorimeter are designed specifically to enhance the environment of small, aqueous quantities for fluorescent analysis. The Teflon coated slides have multiple circles of bare glass that exhibit superhydrophobic properties which allow spherical drops to form on top of the glass. When light from the Blue LED is concentrated on the substance, the intensity excites the SYBR green Dye and causes it to fluoresce. As the dye combines with molecules such as dsDNA, it forms a structure that travels to the surface of the drop for observation. SYBR green fluorescence is a reliable technique because its sensitivity reacts favorably in its environment. | |||
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'''Image J Values for All Samples' | |||
'''Image J Values for All Samples''' | |||
{| {{table}} width=700 | {| {{table}} width=700 | ||
|- | |- | ||
| Concentration || Integrated Density || Integrated Density of Background | | Concentration || Area of Circle || Mean Gray Value || Raw Integrated Density || Raw Integrated Density of Background | ||
|- | |- | ||
|Water|| | | Water||7566||33.153||250834||24944 | ||
|- | |- | ||
|Water|| | | Water||2072||40.972||84895||5326 | ||
|- | |- | ||
|Water|| | | Water||6553||12.891||84473||25857 | ||
|- | |- | ||
|0.25|| | | 0.25||11335||75.733||858434||29401 | ||
|- | |- | ||
|0.25|| | | 0.25||10010||78.333||784117||26530 | ||
|- | |- | ||
|0.25|| | | 0.25||12116||83.009||1005739||44131 | ||
|- | |- | ||
|0.5|| | | 0.5||9964||79.569||792830||25061 | ||
|- | |- | ||
|0.5|| | | 0.5||10260||78.604||806481||28115 | ||
|- | |- | ||
|0.5|| | | 0.5||17391||137.035||2383180||40816 | ||
|- | |- | ||
|1|| | | 1||14512||178.775||2594377||54092 | ||
|- | |- | ||
|1|| | | 1||18151||167.444||3039267||42992 | ||
|- | |- | ||
|1|| | | 1||16108||175.72||2830503||34749 | ||
|- | |- | ||
|2|| | | 2||12623||224.804||2837699||27499 | ||
|- | |- | ||
|2|| | | 2||15887||218.648||3473660||36706 | ||
|- | |- | ||
|2|| | | 2||16880||223.931||3779953||57381 | ||
|- | |- | ||
|5|| | | 5||19160||236.711||4535384||41840 | ||
|- | |- | ||
|5|| | | 5||19612||236.43||4636859||54123 | ||
|- | |- | ||
|5|| | | 5||20035||240.357||4815559||48482 | ||
|- | |- | ||
|} | |} | ||
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'''Fitting a Straight Line'''<br> | '''Fitting a Straight Line'''<br> | ||
[[Image: | [[Image:BME103_Group_2_Lab2Data2Picture.png|500px|thumb|left|]] | ||
<br><font color=red>**PLAGIARISM WARNING: A DUPLICATE VERSION OF THIS IMAGE HAS BEEN FOUND ON ANOTHER TEAM'S LAB REPORT**</font> | |||
Latest revision as of 09:12, 28 June 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye
Single-Drop Fluorimeter
ProcedureSmart Phone Camera Settings
Calibration
These steps were repeated in triplicate for each of the samples listed above.
Data AnalysisRepresentative Images of Samples
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