BME103 s2013:T900 Group1 L2

BME 103 Spring 2013	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Kristi Norris: Protocol Planner Carlos Renteria: Research and Design Specialist Raul Monzolo: Open PCR Machine Engineer Johnny Montez: Open PCR Machine Engineer Robert Sanchez: Research and Design Specialist Group 1 LAB 2 WRITE-UP Background Information SYBR Green Dye SYBR Green dye is a fluorescent dye that binds to double-stranded DNA, but will stain single-stranded DNA with lower performance. Single-Drop Fluorimeter Fluorimeter is a device that is used to measure the amount fluorescence in a single drop. How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet] Procedure Smart Phone Camera Settings We used an Iphone 5 Flash:None ISO setting:NA White Balance:NA Exposure:NA Saturation:NA Contrast:NA No additional phone used Calibration We inserted the Iphone inside a cradle that gave us a right angle view. Afterward we adjusted the height of the phone so that it is as even with the drop. Distance between the smart phone cradle and drop = 3cm Solutions Used for Calibration [See worksheet page 5] row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 [Add more rows as needed] Placing Samples onto the Fluorimeter [Step one, in your OWN words] [Step two, in your own words] [Step three, in your own words] [etc.] Data Analysis Representative Images of Samples Image J Values for All Samples [See worksheet page 5] Concentration Integrated Density Integrated Density of Background Area of Circle Mean Gray Value Water 213454 ?? 6691 31.902 Water 93371 ?? 2422 38.551 Water 65949 ?? 1219 54.101 0.25 593913 ?? 7333 80.992 0.25 569386 ?? 6988 81.481 0.25 791593 ?? 8917 88.773 0.5 651140 ?? 7774 83.759 0.5 453045 ?? 5146 88.038 0.5 2142033 ?? 15458 138.571 1 2458883 ?? 12175 201.962 1 1836054 ?? 9380 195.741 1 2223630 ?? 11335 196.174 2 1721563 ?? 6868 250.664 2 3215604 ?? 12780 251.612 2 2577701 ?? 10205 252.592 5 3957423 ?? 15704 252.001 5 4194978 ?? 16748 250.476 5 5088859 ?? 20200 251.924 [Add more rows as needed] Fitting a Straight Line