BME103:W930 Group9 l2
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Wiki Editing Help
Everyone has contributed to this project even though there are only two usernames. Every person used these two users to make edits to the wiki. Dr. Haynes said that this would be sufficient enough to give each member full participation credit for this project
LAB 2 WRITE-UP
Thermal Cycler Engineering
Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
The KeyPad will be detachable, and will be connected through the USB connection.
The key features of the new design include a larger main heating block and a
The new OpenPCR will be assembled and operated almost identical to the old OpenPCR.
Materials Supplied in the Kit -Newly Designed PCR with additional 2 heating trays -Key Pad attachment -Flourimeter box -Smart phone stand -Teflon coated slides -470 nm wavelength diode laser -100 mL cyber green -15 5 piece test tubes -DNA mixture including the following( this Supplied by the User -Pipets(100) -Smart Phone -Distilled Water -Micro pipets to add the DNA -.2 microliters DNA -
1. Place the flourimeter on the table and turn on the blue light. 2. Place provided glass slide on flourimeter track so that the first row of dots is even with the light. 3. Place phone cradle in front of the flourimeter with a smart phone facing perpendicular to the beam of light. (as seen in the picture). 4. Add two drops of green dye on the dots that are even with the light. 5. Place two drops of DNA sample on top of the green dye. 6. Cover the flourimeter and phone by turning the large box over and placing it above both of them. 7. Take a picture of the droplet with the camera. 8. Save the picture and send it to the imageJ operator. Instructions for opening images in imageJ
1. Take a picture of the fluorimeter assembly with a smartphone. 2. Transfer the picture to a laptop equipped with imageJ via icloud or email. 3. Open imageJ and select file, then open. 4. Find the file on the computer and select it. 5. The image is now open and can be analyzed. 6. The image can be split into three images (blue, green, and red) for better analysis by selecting: image-color-split channels.
Research and Development
Background on Disease Markers