BME103:W930 Group9: Difference between revisions
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| [[Image:tyler.png|100px|thumb|Name: Tyler Ray<br>Research and development scientist]] | | [[Image:tyler.png|100px|thumb|Name: Tyler Ray<br>Research and development scientist]] | ||
| [[Image:BME103_Seth.jpg|100px|thumb|Name: Seth Howell<br> | | [[Image:BME103_Seth.jpg|100px|thumb|Name: Seth Howell<br>Protocol]] | ||
| [[Image:BME103_Ryan.jpg|100px|thumb|Name: Ryan <br>Open PCR machine engineer]] | | [[Image:BME103_Ryan.jpg|100px|thumb|Name: Ryan <br>Open PCR machine engineer]] | ||
| [[Image:Hamas.png|100px|thumb|Name: Hamas<br> | | [[Image:Hamas.png|100px|thumb|Name: Hamas<br>Protocol]] | ||
| [[Image:BME103_Deanna.jpg|100px|thumb|Name: Deanna<br>Open PCR machine engineer]] | | [[Image:BME103_Deanna.jpg|100px|thumb|Name: Deanna<br>Open PCR machine engineer]] | ||
| [[Image:Daniella.jpeg|100px|thumb|Name: Daniela<br>Data Compiler and Analyzer]] | | [[Image:Daniella.jpeg|100px|thumb|Name: Daniela<br>Data Compiler and Analyzer]] |
Revision as of 22:11, 13 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design The open PCR is a device that enables the splitting of DNA. It is run through many cycles of heating and cooling that enables the splitting and recombination with polomers. It connects to a computer program to run the cycles. The temperature change is controled by the heating lid and than the information is fed to the LED screen. The heating lid heats tubes that are held in the heat tube holder to the appropriate temperature and then cools them as needed in each cycle. Experimenting With the Connections When we unplugged part 3, the LCD, from part 6, the Open PCR Brains Board, the LCD on the machine stopped working and did not show anything on it. When we unplugged the white wire that connects part 6, the Open PCR Brains Board, to part 2, the heat tube, the machine no longer measures the temperature of the plate and sends it to the LCD.
First test run: 24 Oct. 2012
ProtocolsPolymerase Chain Reaction The Master Mix
(Add your work from Week 3, Part 2 here) Instructions for opening images in imageJ
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology This report used the technique called DNA amplification. (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
Results
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