BME103:W930 Group8 l2

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BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
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LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in Kit Amount
10 μM Forward Primer 2.5 μL
10 μM Reverse Primer 2.5 μL
GoTaq master mix 119.5 μL
dH2O 119.5 μL
Total Volume 250 μL


Supplied by User Amount
DNA Sample (20ng) .5 μL


Suplied by User Amount
Sample of DNA by user (20ng) .5μL



PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration