BME103:W930 Group8 l2: Difference between revisions
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'''Steps to Run PCR''' | |||
# Plug PCR machine into the computer. | |||
# Open the 'OpenPCR' program on the computer. | |||
# Label the tubes. This information should include the patient number (1 or 2) as well as the replication number. The positive and negative control should also be labeled. | |||
# Prepare the experiment by loading the reactants into the PCR tubes. This will consist of the patients DNA, along with the master mix components. As each tube is filled, put it into the chamber at the top of the machine. | |||
# Close the lid of the chamber. | |||
# Customize the settings in the 'Thermal Cycler' program to include three stages. Stage 1 is one cyle and in it the reactancts will heat up to 95 degrees Celcius for three minutes. During Stage 2, there are 35 cycles. The reactancts will heat to 95 degrees Celsius for 30 seconds, 57 degrees Celsius for 30 seconds, and 72 degrees Celsius for 30 seconds. | |||
# Press start on the program to begin running the PCR. | |||
# Collect and record data at the end of the trial. | |||
Revision as of 20:14, 26 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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