BME103:W930 Group7 l2 - Revision history

Pahul Singh: /* Protocols */

2012-11-28T18:24:37Z

Protocols

←Older revision		Revision as of 18:24, 28 November 2012
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	8. Place this on the fluorometer, making sure the drop is aligned with the light source.		8. Place this on the fluorometer, making sure the drop is aligned with the light source.

-	9. Turn of surrounding lights, then turn on the device and record the results.	+	9. Turn off surrounding lights, then turn on the device and record the results.
		+
		+	10. Take a picture of the drop with the light passing through it with the smartphone. Repeat this for all samples.
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		+	11. Once all the pictures are taken, they must be uploaded into ImageJ as follows:
		+
		+	11a. Obtain a computer and install the ImageJ software.
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		+	11b. After taking the pictures needed, connect the smartphone to the computer with a USB cable.
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		+	11c. Once connected, open the folder containing the smartphone (found in My Computer or Devices).
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		+	11d. Create a folder on the desktop (this will be used to temporarily store the pictures).
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		+	11e. Copy and paste the pictures form the smartphone folder (DCIM) to the desktop one.
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		+	11f. Open the ImageJ software and click File and the Open.
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		+	11g. From this Open menu, select the preferred picture from the folder on the desktop containing the pictures. This will transfer the data into ImageJ

	==Research and Development==		==Research and Development==

2012-11-28T18:19:58Z

Protocols

←Older revision		Revision as of 18:19, 28 November 2012
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	3. Using a fresh pipet, transfer the samples to the new test tubes.		3. Using a fresh pipet, transfer the samples to the new test tubes.

-	4.	+	4. Using a fresh pipet every time, add SYBR Green and the Calf Thymus.
		+
		+	5. Set up the flourometer as follows.
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		+	5a. Remove all contents from the black box.
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		+	5b. After removing the lid, flip the box upside down.
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		+	5c. One side of the box will detach. Fold this side up and create a cave like opening (as seen above).
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		+	5d. Place the Fluorometer device within the upside down box.
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		+	5e. Lastly, place the smartphone in the black stand with the camera facing into the box.
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		+	6. Place the Standardized Mat with Measurements inside the box with the contents on top of it. This ensures that all the tools stay the same distances, allowing for less error and more uniformed results.
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		+	7. Using a fresh pipet, add a drop of the sample and a drop of water to the hydrophobic glass slide.
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		+	8. Place this on the fluorometer, making sure the drop is aligned with the light source.
		+
		+	9. Turn of surrounding lights, then turn on the device and record the results.

	==Research and Development==		==Research and Development==

2012-11-28T18:08:00Z

Protocols

←Older revision		Revision as of 18:08, 28 November 2012
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	1. Once the Open PCR machine has completed its cycles, remove the tubes.		1. Once the Open PCR machine has completed its cycles, remove the tubes.

-	2. Obtain a new set of test tubes	+	2. Obtain a new set of test tubes and label them appropriately.
		+
		+	3. Using a fresh pipet, transfer the samples to the new test tubes.
		+
		+	4.

	==Research and Development==		==Research and Development==

2012-11-28T17:42:48Z

Protocols

2012-11-28T17:40:46Z

Protocols

←Older revision		Revision as of 17:40, 28 November 2012
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	1. Once the Open PCR machine has completed its cycles, remove the tubes.		1. Once the Open PCR machine has completed its cycles, remove the tubes.

-	2.	+	2. Obtain a new set of test tubes

	==Research and Development==		==Research and Development==

2012-11-28T16:38:06Z

Protocols

←Older revision		Revision as of 16:38, 28 November 2012
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	5. In the options menu select 30 number of cycles and the specific temperatures.		5. In the options menu select 30 number of cycles and the specific temperatures.

-	6. Obtain PCR test tubes and label them.	+	6. Obtain PCR test tubes and label them. Put on a lab coat and gloves.

	7. Using the pipet, transfer the DNA into the PCR test tubes.		7. Using the pipet, transfer the DNA into the PCR test tubes.
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	'''DNA Measurement Protocol'''		'''DNA Measurement Protocol'''
		+
		+
		+	1. Once the Open PCR machine has completed its cycles, remove the tubes.
		+
		+	2.

	==Research and Development==		==Research and Development==

2012-11-28T15:39:47Z

Protocols

←Older revision		Revision as of 15:39, 28 November 2012
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	4. Open the Open PCR program and create a new experiment.		4. Open the Open PCR program and create a new experiment.

-	5.	+	5. In the options menu select 30 number of cycles and the specific temperatures.
		+
		+	6. Obtain PCR test tubes and label them.
		+
		+	7. Using the pipet, transfer the DNA into the PCR test tubes.
		+
		+	8. Add a micropipette tip to the micropipette.
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		+	9. Add the primers to the PCR test tubes, making sure to change out the micropipette every time.
		+
		+	10. Then, place the samples (which include the positive and negative controls) into the Open PCR machine.
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		+	11. From the laptop, start the program so the DNA amplification can start.
		+

2012-11-28T08:46:39Z

Protocols

←Older revision		Revision as of 08:46, 28 November 2012
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	3. Connect the laptop and PCR machine using a USB cable.		3. Connect the laptop and PCR machine using a USB cable.
		+
		+	4. Open the Open PCR program and create a new experiment.
		+
		+	5.

2012-11-28T08:39:48Z

Protocols

←Older revision		Revision as of 08:39, 28 November 2012
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	1. Obtain a computer and PCR machine.		1. Obtain a computer and PCR machine.
		+
	2. Download the Open PCR software onto the computer.		2. Download the Open PCR software onto the computer.
		+
	3. Connect the laptop and PCR machine using a USB cable.		3. Connect the laptop and PCR machine using a USB cable.

2012-11-28T08:39:30Z

Protocols

←Older revision		Revision as of 08:39, 28 November 2012
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	'''PCR Protocol'''		'''PCR Protocol'''
-	~~Procedure to run~~ PCR.	+
-	1.	+	1. Obtain a computer and PCR machine.
		+	2. Download the Open PCR software onto the computer.
		+	3. Connect the laptop and PCR machine using a USB cable.