BME103:W930 Group7

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Jake Turner PCR Machine Engineer Name: Tyler Allen PCR Machine Engineer Name: Khalil Pathan Experimental Protocol Planner Name: Pahul Singh Experimental Protocol Planner Name: Frea Mehta Research and Development Specialist Name: Paul Song Research and Development Specialist LAB 1 WRITE-UP Initial Machine Testing The Original Design Write a paragraph description for visitors who have no idea what this is) Experimenting With the Connections When we unplugged part (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Polymerase Chain Reaction (Add your work from Week 3, Part 1 here) Flourimeter Measurements (Add your work from Week 3, Part 2 here) Research and Development Specific Cancer Marker Detection - The Underlying Technology The NCBI database is used to isolate the sequence used and determine specific primers. (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.) Results Sample Integrated Density DNA μg/mL Conclusion PCR: Negative Control 267793 F6 G6 PCR: Positive Control 27409968 F7 G7 PCR: Patient 1 ID #####, rep 1 3511064 F8 G8 PCR: Patient 1 ID #####, rep 2 15099598 F9 G9 PCR: Patient 1 ID #####, rep 3 8451848 F10 G10 PCR: Patient 2 ID #####, rep 1 17311845 E12 G11 PCR: Patient 2 ID #####, rep 2 9289657 E13 G12 PCR: Patient 2 ID #####, rep 3 28825322 F13 G13 KEY Sample = Integrated Density = DNA μg/mL = Conclusion =