BME103:W930 Group6 l2: Difference between revisions
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'''Key Features'''<br> | '''Key Features'''<br> | ||
Our group decided to increase the sample size. We increased it to 48 samples. We designed our Open PCR machine to include this increased sample size. We increased the size of the machine itself to be able to hold 2 more heating plates and lids. We changed the overall shape of the machine to make it wider to include the new heating plates on one of the sides. The controls and the machine itself run in the same way. This would benefit in diagnosing diseases more quickly, especially if a deadly disease is threatening a population. | |||
'''Instructions'''<br> | '''Instructions'''<br> | ||
Building the Heating Lid<br> | |||
1. Peel off the backing paper on all 5 wooden parts. <br> | |||
2. Snap together one of side pieces into the top piece.<br> | |||
3. Pick up a small metal nut. Slide the nut into the “T” cross of the side pieces. (Line up the edges of the nut to be parallel with the cross.)<br> | |||
4. Put a metal screw through the hole. Tighten using your fingers at first. Finish it off with your blue Allen wrench.<br> | |||
5. Repeat steps 2-4 to attach the three other side pieces. <br> | |||
6. When all four wooden sides are attached, then put a metal screw through each hole and tighten.<br> | |||
7. Repeat steps for the other heating lids.<br><br> | |||
Building the Heating Lid Part 2<br> | |||
1. Find the orange lid heater and small rectangular lid plate. Remove the adhesive backing on the orange lid heater. <br> | |||
2. Stick the heater in the middle of the plate. Make sure the orange heater does not hang off the edges, cover up any holes or have any bubbles underneath.<br> | |||
3. Remove the adhesive backing on the white insulation. Stick to the top of the lid heater.<br> | |||
4. Repeat steps for the other heating lids.<br><br> | |||
Building the Heating Lid Part 3 | |||
1. Screw the latch onto the heated lid bracket using 2 short Philips head screws.<br> | |||
2. Screw the hinge to the back of the heated lid bracket with 2 black Philips head screws.<br> | |||
3. Find the small 2 mm black Allen wrench. Place a spring around the shoulder bolt and screw it on to hold the plates together.<br> | |||
4. Repeat with the other 3 shoulder bolts. <br> | |||
5. Repeat steps for the other heating lids.<br> | |||
Revision as of 22:46, 27 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
ProtocolsMaterials Supplied in the Kit ---- Amount Supplied by User-----Amount
Fluorometer Use Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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