Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
System Design
Key Features
The following picture is a simple change in the design of the large exterior screw located on the top of the Open PCR lid:
[IMG:screw.png]
Instructions
Protocols
Materials
Supplied in Kit
Supplies
Amount
Micro Test Tubes
?
Transfer Pipettes
?
PCR Master Mix
6 samples (598.8μL total)
Positive Control Solution*
1 sample (100.0μL***)
Negative Control Solution**
1 sample (100.0μL***)
PCR Machine
1
Fluorimeter
1
(*)Positive control consists of calf thymus DNA
(**)Negative control simply consists of a blank solution of water
(***)Already mixed with PCR master mix
Included in Fluorimeter Package:
Supplies
Amount
Smart phone stand
1
LCD Box
1
Light box
1
Sybr green solution
500.0μL
Components of PCR master mix:
DNA Solution Component
Amount
Patient’s Template DNA*
0.2μL
10μM forward primer
1.0μL
10μM reverse primer
1.0μL
Promega GoTaq master mix
50.0μL
dH2O
47.8μL
Total
100.0μL
(*)Not actually included in kit, but must be added to the master mix by the user.
Supplied by User
Supplies
Amount
Smart Phone with Camera
1
Patient's Template DNA
6 samples (0.2μL each)
External Computer
1
Image J Software
1
Open PCR Software
1
Gloves
1 pair
Lab Coat
1
PCR Protocol
DNA Measurement Protocol
Research and Development
Background on Disease Markers
For this experiment, our group chose to take an in-depth look at acute myeloid leukemia (AML).