Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
System Design
Key Features
Instructions
Protocols
Materials
Supplied in Kit
Supplies
Amount
Micro Test Tubes
?
Transfer Pipettes
?
PCR Master Mix
6 samples (598.8μL total)
Positive Control Solution*
1 sample (100.0μL***)
Negative Control Solution**
1 sample (100.0μL***)
PCR Machine
1
Fluorimeter
1
(*)Positive control consists of calf thymus DNA
(**)Negative control simply consists of a blank solution of water
(***)Already mixed with PCR master mix
Included in Fluorimeter Package
Supplies
Amount
Smart phone stand
1
LCD Box
1
Light box
1
Sybr green solution
500.0μL
Components of PCR master mix:
DNA Solution Component
Amount
Patient’s Template DNA*
0.2μL
10μM forward primer
1.0μL
10μM reverse primer
1.0μL
Promega GoTaq master mix
50.0μL
dH2O
47.8μL
Total
100.0μL
Not actually included in kit, but must be added to the master mix by the user.