BME103:W930 Group5 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Andrea Carpenter Role(s): Experimental Protocol Planner Name: Malik McLaurin Role(s): Open PCR Machine Engineer Name: Dana McElwain Role(s): Open PCR Machine Engineer Name: Chris Anastos Roles(s): R&D Scientist Name: Student Role(s) LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Key Features The following picture is a simple change in the design of the large exterior screw located on the top of the Open PCR lid: [IMG:screw.png] Instructions Protocols Materials Supplied in Kit Supplies Amount Micro Test Tubes ? Transfer Pipettes ? PCR Master Mix 6 samples (598.8μL total) Positive Control Solution* 1 sample (100.0μL***) Negative Control Solution** 1 sample (100.0μL***) PCR Machine 1 Fluorimeter 1 (*)Positive control consists of calf thymus DNA (**)Negative control simply consists of a blank solution of water (***)Already mixed with PCR master mix Included in Fluorimeter Package: Supplies Amount Smart phone stand 1 LCD Box 1 Light box 1 Sybr green solution 500.0μL Components of PCR master mix: DNA Solution Component Amount Patient’s Template DNA* 0.2μL 10μM forward primer 1.0μL 10μM reverse primer 1.0μL Promega GoTaq master mix 50.0μL dH2O 47.8μL Total 100.0μL (*)Not actually included in kit, but must be added to the master mix by the user. Supplied by User Supplies Amount Smart Phone with Camera 1 Patient's Template DNA 6 samples (0.2μL each) External Computer 1 Image J Software 1 Open PCR Software 1 Gloves 1 pair Lab Coat 1 PCR Protocol DNA Measurement Protocol Research and Development Background on Disease Markers Primer Design Illustration