BME103:W930 Group5 l2: Difference between revisions

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'''Materials'''
<u>'''Materials'''</u><br>
<br>
<b>Supplied in the kit</b><br>
<i>Components of PCR master mix:</i>
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''DNA Solution Component'''
| align="center" style="background:#f0f0f0;”|'''Amount'''
|-
| Patient’s Template DNA*||0.2μL
|-
| 10μM forward primer||1.0μL
|-
| 10μM reverse primer||1.0μL
|-
| GoTaq master mix||50.0μL
|-
| dH<sub>2</sub>O||47.8μL
|-
| Total||100.0μL
|}<br>


<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
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'''DNA Measurement Protocol'''
'''DNA Measurement Protocol'''


==Research and Development==
==Research and Development==

Revision as of 22:52, 25 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
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OUR TEAM

Name: Student
Role(s): Experimental Protocol Planner
Name: Student
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Name: Student
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LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the kit
Components of PCR master mix:

DNA Solution Component Amount
Patient’s Template DNA* 0.2μL
10μM forward primer 1.0μL
10μM reverse primer 1.0μL
GoTaq master mix 50.0μL
dH2O 47.8μL
Total 100.0μL


PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers



Primer Design



Illustration