BME103:W930 Group5 l2: Difference between revisions
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| Transfer Pipettes||? | | Transfer Pipettes||? | ||
|- | |- | ||
| | | PCR Master Mix||6 samples (598.8μL total) | ||
|- | |- | ||
| Positive Control Solution||1 sample (100.0μL) | | Positive Control Solution*||1 sample (100.0μL***) | ||
|- | |- | ||
| Negative Control Solution||1 sample (100.0μL) | | Negative Control Solution**||1 sample (100.0μL***) | ||
|- | |- | ||
| PCR Machine||1 | | PCR Machine||1 | ||
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| Fluorimeter||1 | | Fluorimeter||1 | ||
|}<br> | |}<br> | ||
*Positive control consists of calf thymus DNA<br> | |||
**Negative control simply consists of a blank solution of water<br> | |||
***Already mixed with PCR master mix | |||
<i>Included in Fluorimeter Package</i><br> | <i>Included in Fluorimeter Package</i><br> | ||
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| 10μM reverse primer||1.0μL | | 10μM reverse primer||1.0μL | ||
|- | |- | ||
| GoTaq master mix||50.0μL | | Promega GoTaq master mix||50.0μL | ||
|- | |- | ||
| dH<sub>2</sub>O||47.8μL | | dH<sub>2</sub>O||47.8μL | ||
|- | |- | ||
| Total||100.0μL | | Total||100.0μL | ||
|}<br> | |||
*Not actually included in kit, but must be added to the master mix by the user. | |||
<b>Supplied by User</b><br> | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''Supplies''' | |||
| align="center" style="background:#f0f0f0;”|'''Amount''' | |||
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| Smart Phone with Camera||1 | |||
|- | |||
| DNA Solution||6 samples (0.2μL each) | |||
|- | |||
| External Computer||1 | |||
|- | |||
| Image J Software||1 | |||
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| Open PCR Software||1 | |||
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| Gloves||1 pair | |||
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| Lab Coat||1 | |||
|}<br> | |}<br> | ||
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'''PCR Protocol''' | <u>'''PCR Protocol'''</u> | ||
'''DNA Measurement Protocol''' | <u>'''DNA Measurement Protocol'''</u> | ||
==Research and Development== | ==Research and Development== |
Revision as of 23:19, 25 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
Key Features
Instructions
ProtocolsMaterials
Included in Fluorimeter Package
Components of PCR master mix:
Supplied by User
PCR Protocol
Research and DevelopmentBackground on Disease Markers
Primer Design
Illustration
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