BME103:W930 Group4 l2

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Contents

OUR TEAM

Name: Christian VargasProtocol(s)
Name: Christian Vargas
Protocol(s)
Name: Jake KrammerResearch and Development
Name: Jake Krammer
Research and Development
Name: Jan SimperResearch and Development
Name: Jan Simper
Research and Development
Name: Renaad MohammedProtocol(s)
Name: Renaad Mohammed
Protocol(s)
Name: Lauren AllisonOpen PCR Machine Engineer(s)
Name: Lauren Allison
Open PCR Machine Engineer(s)
Name: Justus VangorOpen PCR Machine Engineer(s)
Name: Justus Vangor
Open PCR Machine Engineer(s)

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design

Image:PCR_with_Drawers.PNG

Key Features

Case:

The new casing will become larger to implement a set of two drawers in the front. They will be placed near the top of the case and in the middle of the front panel. These will hold the additional sample plates being added to the design, providing for higher testing numbers.

Ceramic Peltier Heaters:

The new design will have two of these heaters, one corresponding to each drawer of sample plates and placed beneath them, just as they were placed beneath the original heat lid. Adding additional heating elements will allow the machine to heat more samples, more quickly.


Instructions





Protocols

Materials


PCR Protocol



DNA Measurement Protocol



Research and Development

Background on Disease Markers

Our group decided to look at two different diseases, metabolic syndrome and cardiovascular disease. Metabolic syndrome is a name for a group of risk factors that occur together and increase the risk for coronary artery disease, stroke, and type 2 diabetes. Cardiovascular disease refers to any disease that affects the cardiovascular system, principally cardiac disease, vascular diseases of the brain and kidney, and peripheral arterial disease. The associated mutation for metabolic syndrome was rs1800206, and for cardiovascular disease it was rs1801253.



Primer Design

Forward Primer:

Reverse Primer: 5'TCACAAGTGCC3'



Illustration


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