BME103:W930 Group4 l2 - Revision history

Karmella Haynes at 01:37, 30 November 2012

2012-11-30T01:37:04Z

←Older revision		Revision as of 01:37, 30 November 2012
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	{\| style="wikitable" width="700px"		{\| style="wikitable" width="700px"
-	\|-	+	\|- valign="top"
	\| [[Image:Christian_Vargas.jpg\|100px\|thumb\|Name: Christian Vargas<br>Protocol(s)]]		\| [[Image:Christian_Vargas.jpg\|100px\|thumb\|Name: Christian Vargas<br>Protocol(s)]]
	\| [[Image:Jake_Krammer.jpg\|100px\|thumb\|Name: Jake Krammer<br>Research and Development]]		\| [[Image:Jake_Krammer.jpg\|100px\|thumb\|Name: Jake Krammer<br>Research and Development]]

2012-11-28T17:52:57Z

Protocols

2012-11-28T17:51:53Z

Protocols

2012-11-28T17:48:24Z

Protocols

2012-11-28T17:35:56Z

Protocols

2012-11-28T17:35:33Z

Protocols

←Older revision		Revision as of 17:35, 28 November 2012
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	8. use another pipette to transfer GoTaq master mix into the DNA/primer mixture.<br>		8. use another pipette to transfer GoTaq master mix into the DNA/primer mixture.<br>
	9. Dilute the contents of the test tube by filling its remainder with deionized water.<br>		9. Dilute the contents of the test tube by filling its remainder with deionized water.<br>
-	10. Place the 8 test tubes including the positive and negative controls into the Open PCR machine.<br>	+	10. Place the 128 test tubes including the positive and negative controls into the Open PCR machine.<br>
	11. Close the lid <br>		11. Close the lid <br>
	12. Click on “Plug in Open PCR to start” to begin amplifying DNA samples.<br>		12. Click on “Plug in Open PCR to start” to begin amplifying DNA samples.<br>

2012-11-28T17:33:10Z

Protocols

←Older revision		Revision as of 17:33, 28 November 2012
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	<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->		<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
-		+	{\| {{table}}
		+	\|- style="background:#f0f0f0;"
		+	\| '''Supplied in the Kit''' \|\| '''Amount''' \|\|
		+	\|-
		+	\| Template DNA (20 ng) \|\| 0.2 µL \|\|
		+	\|-
		+	\| 10 µM Forward Primer \|\| 1.0 µL \|\|
		+	\|-
		+	\| 10 µM Reverse Primer \|\| 1.0 µL \|\|
		+	\|-
		+	\| GoTaq Master Mix \|\| 50.0 µL \|\|
		+	\|-
		+	\| dH2O \|\| 47.8 µL \|\|
		+	\|-
		+	\| PCR Machine \|\| 1 \|\|
		+	\| PCR Machine \|\| 1 \|\|
		+	\|-
		+	\|}

	'''PCR Protocol'''<br>		'''PCR Protocol'''<br>

2012-11-28T17:28:40Z

Protocols

←Older revision		Revision as of 17:28, 28 November 2012
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-	'''DNA Measurement Protocol'''	+	'''DNA Measurement Protocol'''<br>
	1. Open the lid of the PCR machine (by releasing the catch) and remove the 8 samples from the PCR machine.<br>		1. Open the lid of the PCR machine (by releasing the catch) and remove the 8 samples from the PCR machine.<br>
	2. Use multiple pipettes to put two drops of dye and two drops of the sample on a glass slide. <br>		2. Use multiple pipettes to put two drops of dye and two drops of the sample on a glass slide. <br>

2012-11-28T17:27:48Z

Protocols

←Older revision		Revision as of 17:27, 28 November 2012
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	10. Using the android phone, email the pictures taken from the phone to the email of the ImageJ Software Operator. <br>		10. Using the android phone, email the pictures taken from the phone to the email of the ImageJ Software Operator. <br>
	11. Open the email of the ImageJ Operator and save the pictures onto the computer of the ImageJ Software Operator. <br>		11. Open the email of the ImageJ Operator and save the pictures onto the computer of the ImageJ Software Operator. <br>
		+	12. Open up the ImageJ Software. <br>
		+	13. Click File > Open. <br>
		+	14. Then select the images from wherever they were saved on the computer. <br>
		+	15. Click open and the image should appear in the program.<br>

	==Research and Development==		==Research and Development==

2012-11-28T17:27:16Z

Protocols

←Older revision		Revision as of 17:27, 28 November 2012
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	5. Turn on the blue LED light. <br>		5. Turn on the blue LED light. <br>
	6. Place the smartphone on the holder provided and position them in front of the device. <br>		6. Place the smartphone on the holder provided and position them in front of the device. <br>
		+	7. Place a box on top of the device and smartphone to create a dark environment. <br>
		+	8. Take a close clear picture of the drop. <br>
		+	9. 8)Make sure the pictures are clear by turning off the flash and placing the smartphone holder as close as possible to the device also make sure the phone is at the same position each time.<br>
		+	10. Using the android phone, email the pictures taken from the phone to the email of the ImageJ Software Operator. <br>
		+	11. Open the email of the ImageJ Operator and save the pictures onto the computer of the ImageJ Software Operator. <br>

	==Research and Development==		==Research and Development==