BME103:W930 Group3 l2

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(Protocols)
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==Protocols==
==Protocols==
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<!--- Design a new protocol based on your group's new PCR design. Make a step-by-step list of how someone should use your method
 
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Things to consider:
 
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How should the PCR machine be set up? Does it need to be plugged in?
 
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How many samples will fit into a single 2-hour run?
 
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How many replicates should be created per patient?
 
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What should the final volume of the reaction be?
 
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Will signal reading be integrated into the PCR machine or remain separate?
 
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If it is separate, you will need to include instructions on how to use the fluorimeter.
 
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How should the user calculate the about of signal amplified?
 
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etc.
 
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--->
 
'''Materials'''
'''Materials'''
 +
'''List of Required Materials for PCR & DNA measurements'''
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<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
 
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{| {{table}}
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|- style="background:#f0f0f0;"
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| '''Supplied in the Kit''' || '''Amount'''
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|-
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| Cyber-Green 1 Dye diluted with buffer || ~~
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|-
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| 2 || ~~
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|-
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| 3 || ~~
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|-
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| 4 || ~~
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|-
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| 5 || ~~
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|-
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| 6 || ~~
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|-
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| 7 || ~~
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|-
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| 8 || ~~
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|-
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| 9 || ~~
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|-
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| 10 || ~~
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|-
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| 11 || ~~
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|-
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| 12 || ~~
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|}
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{| {{table}}
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|- style="background:#f0f0f0;"
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| '''Supplied by User''' || '''Amount'''
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|-
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| 1 || ~~
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|-
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| 2 || ~~
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|-
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| 3 || ~~
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|-
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| 4 || ~~
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|-
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| 5 || ~~
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|-
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| 6 || ~~
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|-
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| 7 || ~~
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|-
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| 8 || ~~
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|-
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| 9 || ~~
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|-
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| 10 || ~~
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|-
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| 11 || ~~
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|-
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| 12 || ~~
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|}
'''PCR Protocol'''
'''PCR Protocol'''
 +
Step 1)
 +
Step 2)
 +
Step 3)
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 +
Step 4)
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 +
Step 5)
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 +
Step 6)
 +
 +
Step 7)
'''DNA Measurement Protocol'''
'''DNA Measurement Protocol'''
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Step 1)
 +
 +
Step 2)
 +
 +
Step 3)
 +
 +
Step 4)
 +
 +
Step 5)
 +
Step 6)
 +
Step 7)
 +
Step 8)
==Research and Development==
==Research and Development==

Revision as of 01:04, 28 November 2012

BME 103 Fall 2012 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png

Contents

Our Team

Name: Ryan BathRole(s) Open PCR Machine Engineer
Name: Ryan Bath
Role(s) Open PCR Machine Engineer
Name: Geon-Woo KimRole(s) Experimental Protocol Planner
Name: Geon-Woo Kim
Role(s) Experimental Protocol Planner
Name: Troy KozlowskiRole(s) Open PCR Machine Engineer
Name: Troy Kozlowski
Role(s) Open PCR Machine Engineer
Name: Phillip MercadoRole(s) Experimental Protocol Planner
Name: Phillip Mercado
Role(s) Experimental Protocol Planner
Name: Eliza NormenRole(s) R&D Scientist
Name: Eliza Normen
Role(s) R&D Scientist
Name: Jacob SwartzRole(s) R&D Scientist
Name: Jacob Swartz
Role(s) R&D Scientist

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design
Image:lidchanges.png


New Lid Catch
Image:catch.png


Key Features
The redesigned version of the Open PCR system focused on resolving the issues that surrounded the function of the Open PCR machine's lid. One modification made was the changing of materials used in production of the PCR lid. In the original design of this machine wood was the main structural material of the Open PCR's lid; however,this redesign changes the material used on the sides of the lid from wood to plexi-glass. The use of plexi-glass allows for the user to see into the lid of the Open PCR machine.In turn allowing the user to view the height of the heat plate while adjusting it, this then minimizes the the chance of the user crushing the DNA samples while lowering the heat plate. The other modification present in the redesign was that the magnetic clasp from the original design was replace by spring catch. The original magnetic clasp on the PCR required too much force to open. While this magnet prevented the lid to open mistakenly during an experiment, it made the machine vulnerable to breakage when the lid needed to be opened. The newly added catch provides the same amount of protection in terms of mistaken lid opening but it also increases ease of use and limits the chance of breakage while opening the lid.


Instructions





Protocols

Materials List of Required Materials for PCR & DNA measurements


Supplied in the Kit Amount
Cyber-Green 1 Dye diluted with buffer ~~
2 ~~
3 ~~
4 ~~
5 ~~
6 ~~
7 ~~
8 ~~
9 ~~
10 ~~
11 ~~
12 ~~
Supplied by User Amount
1 ~~
2 ~~
3 ~~
4 ~~
5 ~~
6 ~~
7 ~~
8 ~~
9 ~~
10 ~~
11 ~~
12 ~~

PCR Protocol

Step 1)

Step 2)

Step 3)

Step 4)

Step 5)

Step 6)

Step 7)

DNA Measurement Protocol Step 1)

Step 2)

Step 3)

Step 4)

Step 5)

Step 6)

Step 7)

Step 8)

Research and Development

Background on Disease Markers



Primer Design



Illustration


Personal tools