BME103:W930 Group2 l2

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BME 103 Fall 2012 Home
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Lab Write-Up 1
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OUR TEAM

Name: Matt Mortensen
Machine Engineer
Name: Student
Role(s)
Name: Student
Role(s)
Name: Student
Role(s)
Name: Student
Role(s)

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features
Clearly our group was more focused on correcting the lid-opening system for the top of the lid to the box. We focused on designing a new hook-and-latch system for the lid because we felt that the jerky nature of the current system was very inconvenient to the user. Whereas the current model is more difficult to open and yanking it can create potential for harm to be done to the DNA samples, our new design corrects for this by allowing means for a smooth and easy opening motion, and the samples are still protected because the lid is secured by a hook when closed. Considering that our other modification of making the sides thicker was a means to make assembling the device easier, it is reasonable to conclude that overall, we were focused on a greater convenience for the user by making a more user-friendly device.


Instructions
Altered instructions are only needed for the new hook-and-latch system: Instead of instructions to screw the Strike into the top, there will only be instructions to insert the hook into the latch. There will already be another hook for it to catch on the bottom of the lid. There should also be a note for contact information in the event that the hook was damaged or lost in the shipping process.




Protocols

Materials


PCR Protocol



DNA Measurement Protocol



Research and Development

Background on Disease Markers



Primer Design



Illustration