BME103:W930 Group2 l2: Difference between revisions
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| [[Image:MattMortensen.jpg|100px|thumb|Name: Matt Mortensen<br>Open PCR Machine Engineer]] | | [[Image:MattMortensen.jpg|100px|thumb|Name: Matt Mortensen<br>Open PCR Machine Engineer]] | ||
| [[Image:Garrett-BME103-Group2.jpg|100px|thumb|Name: Garrett Salcido<br>Open PCR machine engineer]] | | [[Image:Garrett-BME103-Group2.jpg|100px|thumb|Name: Garrett Salcido<br>Open PCR machine engineer]] | ||
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=LAB 2 WRITE-UP= | =LAB 2 WRITE-UP= | ||
<br>DISCLAIMER: ALL GROUP MEMBERS PARTICIPATED EQUALLY IN THE WRITING OF REPORTS. SOME OF US HAD ISSUES LOGGING IN SO CONTRIBUTIONS MAY NOT APPEAR TO ADD UP APPROPRIATELY. | |||
==Thermal Cycler Engineering== | ==Thermal Cycler Engineering== |
Latest revision as of 17:06, 10 December 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UP
Thermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
ProtocolsMaterials
Step 1: Denaturation by Heat
Turn on the excitation light on the fluorimeter. Put a smart phone in the cradle and set it up to take pictures of the slide. Place two drops of water in the middle of the first two rows of the slide using a pipette. Align the drop by moving the slide so the drop is in the middle of the black fiber optic fitting. Cover the fluorimeter with the light box while maintaining the ability to use the smart phone to take pictures.
Research and DevelopmentBackground on Disease Markers Crohn's disease is a chronic, autoimmune inflammatory bowel disease (IBD) that causes inflammation of the digestive tract, also known as the gastrointestinal (GI) tract. Chronic, unmanaged inflammation can lead to a variety of symptoms. Researchers have also discovered genetic variations in certain regions of chromosome 5 and chromosome 10 that appear to contribute to Crohn disease risk. One area of chromosome 5, known as the IBD5 locus, contains several genetic changes that may increase the risk of developing this condition. Other regions of chromosome 5 and chromosome 10 identified in studies of Crohn disease risk are known as "gene deserts" because they include no known genes. The SNP's that are associated with this disease are rs11805303, rs10210302, rs9858542 in the BSN gene, rs17234657, rs1000113, rs10761659 and many more.- www.snpedia.com
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