BME103:W930 Group1 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Kevin Chu Experimemtal Protocol Planner Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Key Features Instructions Protocols Materials Supplied in the Kit Amount PCR Machine 1 Fluorimeter 1 Patient’s Template DNA 0.1 Positive Control TBD Negative Control TBD 10μM forward primer 0.5 10μM reverse primer 0.5 GoTaq master mix 25.0μL dH2O 23.9μL Transfer Pipettes TBD Tubes TBD 0.025% Tris Buffer TBD Supplied by the User Amount Smartphone 1 Computer 1 Open PCR Software 1 ImageJ Software 1 Sharpie (fine point) 1 PCR Protocol DNA Measurement Protocol Research and Development Background on Disease Markers Heterotaxty (Hetero-different) (taxy-arrangement) syndrome is an uncommon birth defect that primary occurs in the heart. This disease can also occur in other organs but it is less likely. In this syndrome, organs that are paired together have a mirror image of each other instead of having their own charcterstics. Other organs can also be arranged This is not the only kind of the Heteotaxy however. As previously stated, the more common defects are located in the heart. Since most of the defects occur at birth, there is a varying type and severity. Primer Design Illustration