BME103:W930 Group1 l2

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'''Background on Disease Markers'''
'''Background on Disease Markers'''
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<!--- A description of the diseases and their associated SNP's (include the database reference number and web link) --->
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<!--- A description of the diseases and their associated SNP's (include the database reference number and web link) --->Heterotaxty (Hetero-different) (taxy-arrangement) syndrome is an uncommon birth defect that primary occurs in the heart. This disease can also occur in other organs but it is less likely. In this syndrome, organs that are paired together have a mirror image of each other instead of having their own charcterstics. Other organs can also be arranged  This is not the only kind of the Heteotaxy however. As previously stated, the more common defects are located in the heart. Since most of the defects occur at birth, there is a varying type and severity.

Revision as of 18:03, 25 November 2012

BME 103 Fall 2012 Home
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Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
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Contents

OUR TEAM

Name: Kevin ChuExperimemtal Protocol Planner
Name: Kevin Chu
Experimemtal Protocol Planner
Name: StudentRole(s)
Name: Student
Role(s)
Name: StudentRole(s)
Name: Student
Role(s)
Name: StudentRole(s)
Name: Student
Role(s)
Name: StudentRole(s)
Name: Student
Role(s)
Name: StudentRole(s)
Name: Student
Role(s)
Name: StudentRole(s)
Name: Student
Role(s)

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.


System Design


Key Features


Instructions





Protocols

Materials

Supplied in the Kit Amount
PCR Machine1
Fluorimeter1
Patient’s Template DNA0.1
Positive ControlTBD
Negative ControlTBD
10μM forward primer0.5
10μM reverse primer0.5
GoTaq master mix25.0μL
dH2O23.9μL
Transfer PipettesTBD
TubesTBD
0.025% Tris BufferTBD

Supplied by the User Amount
Smartphone1
Computer1
Open PCR Software1
ImageJ Software1
Sharpie (fine point)1

PCR Protocol



DNA Measurement Protocol

Research and Development

Background on Disease Markers

Heterotaxty (Hetero-different) (taxy-arrangement) syndrome is an uncommon birth defect that primary occurs in the heart. This disease can also occur in other organs but it is less likely. In this syndrome, organs that are paired together have a mirror image of each other instead of having their own charcterstics. Other organs can also be arranged This is not the only kind of the Heteotaxy however. As previously stated, the more common defects are located in the heart. Since most of the defects occur at birth, there is a varying type and severity.



Primer Design



Illustration


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