BME103:W930 Group1 l2

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Kevin Chu Experimemtal Protocol Planner Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) Name: Student Role(s) LAB 2 WRITE-UP Thermal Cycler Engineering Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski. System Design Key Features Instructions Protocols Materials Supplied in the Kit Amount PCR Machine 1 Fluorimeter 1 Patient’s Template DNA 0.1 Positive Control TBD Negative Control TBD 10μM forward primer 0.5 10μM reverse primer 0.5 GoTaq master mix 25.0μL dH2O 23.9μL Transfer Pipettes TBD Tubes TBD 0.025% Tris Buffer TBD Supplied by the User Amount Smartphone 1 Computer 1 Open PCR Software 1 ImageJ Software 1 Sharpie (fine point) 1 PCR Protocol DNA Measurement Protocol Research and Development Background on Disease Markers Primer Design Illustration