BME103:W930 Group10 l2: Difference between revisions
Line 139: | Line 139: | ||
'''DNA Measurement Protocol''' | '''DNA Measurement Protocol''' | ||
ImageJ Procedure:<br> | |||
1. Open up the image. | |||
2. Go to Set Measurement under the tab analyze, and select area, integrated density, and mean grey value. After doing this once, the setting should remain the same. | |||
3. Split the channels, by going under the tab Image, and then Color. This will split the file into three files, one of which will be labeled as a green channel. | |||
4. Select the green image and then select the oval selection tool. | |||
5. Draw an oval around the droplet in the image and select Measure under the Analyze tab. | |||
6. The same oval can me moved to the background of the image. Then select Measure. | |||
7. Record all values, or save them as an excel file in ImageJ. | |||
8. Repeat all steps for each image. | |||
==Research and Development== | ==Research and Development== |
Revision as of 01:41, 28 November 2012
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 2 WRITE-UPThermal Cycler EngineeringOur re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.
PCR Block Key Features Heat Sink/Fan/etc.: These pieces will be enlarged accordingly with the other parts of the device as necessary for the additional samples. The placement may also be shifted for this accommodation with smaller class sizes. Benefit and Function: Since this device is aimed at being educational, an important factor is price. It will be cheaper to use a larger device to accomodate more students. Instructions
ProtocolsMaterials
PCR Protocol
Flourimeter Setup: DNA Measurement Protocol ImageJ Procedure: Research and DevelopmentBackground on Disease Markers There are two diseases that our group decided to look into. The first disease is type II diabetes, which is the most common of the diabetes. The body does not produce enough insulin, which regulates the use of glucose, therefore leading to high levels of glucose in the blood. There is a mutation in the third chromosome which causes this disease. The SNP related to this is rs4402960 [1]. The second disease is insomnia. This is a sleeping disorder in which a person cannot fall asleep or stay asleep for as long as they would like. It is caused by a mutation in the twentieth chromosome. The SNP related to this is rs74315403 [2].
|