OUR TEAM

Name: Susan Sajadi Role(s)

Name: Rachel Lundeen Role(s)Research and Development

Name: Elizabeth Lopez Role(s)

Name: Britny Sepulveda Role(s)

Name: Raymond Feliciano Role(s)

Name: Colin Siguenza Role(s)

Name: Rotem Beger Role(s)

Name: Lars Moss Role(s)

LAB 2 WRITE-UP

Thermal Cycler Engineering

Our re-design is based upon the Open PCR system originally designed by Josh Perfetto and Tito Jankowski.

System Design

Key Features
The design includes a color coordinated tray and a larger size in order to make Open PCR easier for a class of students.

Instructions

Protocols

Materials

PCR Protocol

DNA Measurement Protocol

Research and Development

Background on Disease Markers

There are two diseases that our group decided to look into. The first disease is type II diabetes, which is the most common of the diabetes. The body does not produce enough insulin, which regulates the use of glucose, therefore leading to high levels of glucose in the blood.

Primer Design

Forward Primer for Type 2 Diabetes:
GGACAGTAGATT[G/T]AAGATACTGATTGTGTTTGCAAACA
Reverse Primer for Type 2 Diabetes:
GGGCATGTTTGCAAACACAATCAGTATCTTAATCTACTGTCC
Forward Primer for Insomnia:
ACAGCAACCAGAACAACTTTGTGCAC[A/G]ACTGCGTCAATATCACAATCAAGCA
Reverse Primer for Insomnia:

Illustration