BME103:TEMPwu2 - Revision history

Karmella Haynes: /* OUR TEAM */

Karmella Haynes — Fri, 16 Nov 2012 20:24:56 GMT

OUR TEAM

←Older revision		Revision as of 20:24, 16 November 2012
Line 12:		Line 12:

	{\| style="wikitable" width="700px"		{\| style="wikitable" width="700px"
-	\|-	+	\|- valign="top"
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Student<br>Role(s)]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Student<br>Role(s)]]
	\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Student<br>Role(s)]]		\| [[Image:BME103student.jpg\|100px\|thumb\|Name: Student<br>Role(s)]]

Karmella Haynes: /* Research and Development */

Karmella Haynes — Thu, 15 Nov 2012 17:44:56 GMT

Research and Development

←Older revision		Revision as of 17:44, 15 November 2012
Line 75:		Line 75:
	==Research and Development==		==Research and Development==

-	<!--- ~~Explain~~ how ~~PCR will~~ be used to ~~amplify signal from your target~~ of ~~choice and why~~ the test ~~will produce no signal if the target is absent~~ --->	+	<!--- Bonus: explain how Bayesian statistics can be used to assess the reliability of your team's method. Just write the equation using variables that are relevant to your team's new test. You do not need actual numbers --->

-	<!--- Explain how Bayesian statistics can be used to assess the reliability of your team's method. Just write the equation using variables that are relevant to your team's new test. You do not need actual numbers --->

		+	'''Background on Disease Markers'''

		+	<!--- A description of the diseases and their associated SNP's (include the database reference number and web link) --->
		+
		+
		+
		+
		+	'''Primer Design'''
		+
		+	<!--- Include the sequences of your forward and reverse primers. Explain why a disease allele will give a PCR product and the non-disease allele will not. --->
		+
		+
		+
		+
		+	'''Illustration'''
		+
		+	<!--- Include an illustration that shows how your system's primers allow specific amplification of the disease-related SNP --->


	<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
	\|}		\|}

Karmella Haynes: /* LAB 2 WRITE-UP */

Karmella Haynes — Wed, 14 Nov 2012 19:32:30 GMT

LAB 2 WRITE-UP

←Older revision		Revision as of 19:32, 14 November 2012
Line 47:		Line 47:

	<!--- Design a new protocol based on your group's new PCR design. Make a step-by-step list of how someone should use your method		<!--- Design a new protocol based on your group's new PCR design. Make a step-by-step list of how someone should use your method
-
	Things to consider:		Things to consider:
	How should the PCR machine be set up? Does it need to be plugged in?		How should the PCR machine be set up? Does it need to be plugged in?
Line 59:		Line 58:
	--->		--->

-	'''~~Example List~~'''	+	'''Materials'''
-	~~# Step 1~~	+
-	~~# Step 2~~	+	<!--- Place your two tables "Supplied in the kit" and "Supplied by User" here --->
-	~~# Step 3~~	+
		+
		+	'''PCR Protocol'''
		+
		+
		+
		+
		+	'''DNA Measurement Protocol'''
		+
		+

Karmella Haynes: /* Thermal Cycler Engineering */

Karmella Haynes — Wed, 14 Nov 2012 18:35:46 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 18:35, 14 November 2012
Line 29:		Line 29:

	'''System Design'''<br>		'''System Design'''<br>
		+
		+

	'''Key Features'''<br>		'''Key Features'''<br>
		+
		+

	'''Instructions'''<br>		'''Instructions'''<br>
		+

	<!--- From Week 4 exercise --->		<!--- From Week 4 exercise --->

Karmella Haynes at 18:35, 14 November 2012

Karmella Haynes — Wed, 14 Nov 2012 18:35:04 GMT

←Older revision		Revision as of 18:35, 14 November 2012
Line 28:		Line 28:


-	'''~~Our Proposed~~ Design'''<br>	+	'''System Design'''<br>

-	<!--- ~~Add results from~~ Week 3 exercise ~~Part 2~~ --->	+	'''Key Features'''<br>
		+
		+	'''Instructions'''<br>
		+
		+	<!--- From Week 4 exercise --->

Karmella Haynes: /* LAB 2 WRITE-UP */

Karmella Haynes — Fri, 09 Nov 2012 09:07:06 GMT

LAB 2 WRITE-UP

←Older revision		Revision as of 09:07, 9 November 2012
Line 21:		Line 21:

	=LAB 2 WRITE-UP=		=LAB 2 WRITE-UP=
-	~~(Please finish by 11/28/2012)~~

Karmella Haynes at 09:06, 9 November 2012

Karmella Haynes — Fri, 09 Nov 2012 09:06:18 GMT

←Older revision		Revision as of 09:06, 9 November 2012
Line 38:		Line 38:
	==Protocols==		==Protocols==

-	~~'''Polymerase Chain Reaction'''~~<~~br>~~	+	<!--- Design a new protocol based on your group's new PCR design. Make a step-by-step list of how someone should use your method
-	~~(Add~~ your ~~work from Week 3, Part 1 here)<br>~~	+

		+	Things to consider:
		+	How should the PCR machine be set up? Does it need to be plugged in?
		+	How many samples will fit into a single 2-hour run?
		+	How many replicates should be created per patient?
		+	What should the final volume of the reaction be?
		+	Will signal reading be integrated into the PCR machine or remain separate?
		+	If it is separate, you will need to include instructions on how to use the fluorimeter.
		+	How should the user calculate the about of signal amplified?
		+	etc.
		+	--->

-	'''~~Flourimeter~~'''	+	'''Example List'''
		+	# Step 1
		+	# Step 2
		+	# Step 3


Line 49:		Line 61:
	<!--- Explain how PCR will be used to amplify signal from your target of choice and why the test will produce no signal if the target is absent --->		<!--- Explain how PCR will be used to amplify signal from your target of choice and why the test will produce no signal if the target is absent --->

-	~~==Results==~~	+	<!--- Explain how Bayesian statistics can be used to assess the reliability of your team's method. Just write the equation using variables that are relevant to your team's new test. You do not need actual numbers --->

-	~~(Your group will add the results of your Fluorimeter measurements here after Week 4)~~

Karmella Haynes: /* Research and Development */

Karmella Haynes — Fri, 09 Nov 2012 08:56:15 GMT

Research and Development

←Older revision		Revision as of 08:56, 9 November 2012
Line 47:		Line 47:
	==Research and Development==		==Research and Development==

		+	<!--- Explain how PCR will be used to amplify signal from your target of choice and why the test will produce no signal if the target is absent --->

	==Results==		==Results==

Karmella Haynes: /* Thermal Cycler Engineering */

Karmella Haynes — Fri, 09 Nov 2012 08:51:38 GMT

Thermal Cycler Engineering

←Older revision		Revision as of 08:51, 9 November 2012
Line 30:		Line 30:

	'''Our Proposed Design'''<br>		'''Our Proposed Design'''<br>
-	(Add results from Week 3 exercise Part 2)	+
		+	<!--- Add results from Week 3 exercise Part 2 --->


	<br><br>		<br><br>
		+
	==Protocols==		==Protocols==

Karmella Haynes: New page: {|{{table}} width="800" |- |style="background-color: #EEE"|128px BME 103 Fall 2012 |style="background-color: #F2F2F2" | ...

Karmella Haynes — Wed, 31 Oct 2012 06:50:07 GMT

New page: {|{{table}} width="800" |- |style="background-color: #EEE"|128px BME 103 Fall 2012 |style="background-color: #F2F2F2" | ...

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]] BME 103 Fall 2012
|style="background-color: #F2F2F2" | [[BME103 | '''Home''' ]] [[BME103:People | '''People''' ]] [[BME103:Projects | '''Lab Write-Up 1''' ]] [[BME103:Projects2 | '''Lab Write-Up 2''' ]] [[BME103:Projects3 | '''Lab Write-Up 3''' ]] [[BME103:Logistics | ''' Course Logistics For Instructors''' ]] [[BME103:Photos | '''Photos''' ]] [[BME103:WikiHelp | '''Wiki Editing Help''' ]]
|-
| style="background-color: #ffcc66; padding: 5px;" colspan="2" | [[Image:BME494_Asu_logo.png]]
|-
| colspan="2"|


=OUR TEAM=

{| style="wikitable" width="700px"
|-
| [[Image:BME103student.jpg|100px|thumb|Name: Student Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: Student Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: Student Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: Student Role(s)]]
| [[Image:BME103student.jpg|100px|thumb|Name: Student Role(s)]]
|}

=LAB 2 WRITE-UP=
(Please finish by 11/28/2012)

==Thermal Cycler Engineering==

Our re-design is based upon the [http://openpcr.org Open PCR] system originally designed by Josh Perfetto and Tito Jankowski. 

'''Our Proposed Design''' 
(Add results from Week 3 exercise Part 2)

 
==Protocols==

'''Polymerase Chain Reaction''' 
(Add your work from Week 3, Part 1 here) 

'''Flourimeter'''

==Research and Development==

==Results==

(Your group will add the results of your Fluorimeter measurements here after Week 4)


|}